Bioassay-guided isolation and evaluation of anti-osteoporotic polysaccharides from Morinda officinalis.

Journal of Ethnopharmacology
Dawei ZhangChunyan Yan

Abstract

Morinda officinalis is a well-known Chinese tonic herb that has shown clinical efficacy in the treatment of bone disease. However, its anti-osteoporotic potential and the M. officinalis polysaccharides (MOPs) responsible for activity require further investigation. This study aimed to investigate the anti-osteoporotic effects of different MOP fractions in ovariectomized (OVX) rats, and to identify the osteoprotective components by bioassay-guided isolation. MOPs were prepared by hot water and alkali extraction, separated into three fractions (MO50, MO70, and MOB) and evaluated in the classic OVX rat model and in MC3T3-E1 cells for anti-osteoporotic activity. Administration of MOPs (400 mg/kg/day) provided significant protection against ovariectomy-induced bone loss and biomechanical dysfunction in rats. Treated animals exhibited reduced deterioration of trabecular microarchitecture and lower levels of bone turnover markers. Bioactivity-guided fractionation led to the isolation of two inulin-type fructans from MO50, MOW50-1 and MOP50-2, with potential anti-osteoporotic activities. These consisted of (2 → 1)-linked β-D-fructosyl residues with degrees of polymerization (DP) of 7 and 13, respectively. Furthermore, MOW50-1 promoted o...Continue Reading

References

Nov 15, 2000·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·A LiesegangM Wanner
May 10, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Vaida GlattMary L Bouxsein
Jul 22, 2008·International Journal of Biological Macromolecules·Zhu MengYongFen JianMin
Jun 11, 2009·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nan LiHong Zhang
Jun 10, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Mary L BouxseinRalph Müller
Dec 21, 2010·Phytochemistry·Puthanapura M ChandrashekarYeldur P Venkatesh
Sep 29, 2011·Chemico-biological Interactions·Leilei BaoQiaoyan Zhang
Aug 7, 2012·Nature Protocols·Filomena A PettolinoAntony Bacic
Feb 23, 2013·Biochimica Et Biophysica Acta·Gordon M Cragg, David J Newman
Aug 19, 2015·Biotechnology Advances·Atanas G AtanasovHermann Stuppner
Aug 21, 2015·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Gisela Rodrigues da Silva SassoManuel de Jesus Simões
Mar 24, 2016·World Journal of Orthopedics·Paola PisaniSergio Casciaro
Apr 21, 2017·Archives of Osteoporosis·J CompstonUNKNOWN National Osteoporosis Guideline Group (NOGG)
Jun 10, 2017·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Ewelina WiercigrochKamilla Malek
Aug 21, 2018·International Journal of Biological Macromolecules·Keming JiangChunyan Yan
Nov 6, 2018·Biochemical and Biophysical Research Communications·Yu-Qiong HeQiao-Yan Zhang
Aug 3, 2019·Bone Reports·Fatme Al AnoutiHabiba Alsafar
Aug 6, 2019·International Journal of Biological Macromolecules·Dawei ZhangChunyan Yan

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.