Bioassay-guided isolation of anti-inflammatory and antinociceptive compound from Plumbago zeylanica leaf

Pharmaceutical Biology
E SheejaD C Jain

Abstract

Plumbago zeylanica Linn. (Plumbaginaceae) is used in the treatment of various inflammatory ailments in traditional medicines. In order to validate these ethnobotanical practices, the anti-inflammatory and antinociceptive activities of various leaf extracts (petroleum ether (60-80 degrees ), chloroform, acetone, ethanol, and aqueous) were studied using in vivo experimental models at two dose levels (200 and 400 mg/kg, p.o.). Anti-inflammatory activity was tested using the carrageenan induced rat hind paw edema method while analgesic activity was studied using the hot plate and formalin induced models. Diclofenac (100 mg/ kg) was used as the reference standard in both anti-inflammatory and analgesic models and morphine (10 mg/ kg, i.p.) was used as the reference standard in the formalin induced analgesic model. The acetone extract significantly (p < 0.01) reduced inflammation in the rats when compared to the control group. As for the analgesia effect, the acetone and petroleum ether extracts significantly (p < 0.01) decreased the pain stimulus only in the later phase of the formalin test, suggesting that the drug could be peripherally acting. Bioassay-guided fractionation of the acetone extract led to the isolation and identifica...Continue Reading

References

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Citations

Dec 16, 2011·The Journal of Alternative and Complementary Medicine : Research on Paradigm, Practice, and Policy·Mohammed RahmatullahRownak Jahan
Jun 2, 2012·Journal of Pharmacological and Toxicological Methods·Chellampillai BothirajaKarimunnisa S Shaikh
Dec 4, 2013·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Tingyu WangTingting Tang
May 6, 2015·Cell Stress & Chaperones·Shuai ZhangTing-Bin Yan
Sep 23, 2010·The Journal of Pharmacology and Experimental Therapeutics·Pei LuoHua Zhou
Apr 15, 2016·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Shi-Xun WangJing-Quan Zhong

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