Bioassay-guided isolation of three anthelmintic compounds from Warburgia ugandensis Sprague subspecies ugandensis, and the mechanism of action of polygodial

International Journal for Parasitology
Maoxuan LiuWalter Luyten

Abstract

Parasitic helminths continue to pose problems in human and veterinary medicine, as well as in agriculture. Resistance to current anthelmintics has prompted the search for new drugs. Anthelmintic metabolites from medicinal plants could be good anthelmintic drug candidates. However, the compounds active against nematodes have not been identified in most medicinal plants with anthelmintic activity. In this study, we aimed to identify the active compounds against helminths in Warburgia ugandensis Sprague subspecies ugandensis (Canellaceae) and study the underlying mechanism of action. A bioassay-guided isolation of anthelmintic compounds from the plant was performed using a Caenorhabditis elegans (C. elegans) test model with a WMicrotracker instrument to monitor motility. Three active compounds were purified and identified by nuclear magnetic resonance and high resolution MS: warburganal (IC50: 28.2 ± 8.6 μM), polygodial (IC50: 13.1 ± 5.3 μM) and alpha-linolenic acid (ALA, IC50: 70.1 ± 17.5 μM). A checkerboard assay for warburganal and ALA as well as polygodial and ALA showed a fractional inhibitory concentration index of 0.41 and 0.37, respectively, suggesting that polygodial and ALA, as well as warburganal and ALA, have a synergi...Continue Reading

Citations

Jun 19, 2019·Parasites & Vectors·Jose F Garcia-BustosRobin B Gasser
Jul 18, 2020·Biomolecules·Sujogya Kumar PandaWalter Luyten
Jan 23, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Muhammad Dawood ShahBalu Alagar Venmathi Maran
Jun 3, 2021·International Journal of Molecular Sciences·Purity N KipangaWalter Luyten
May 25, 2021·Veterinary Parasitology : Regional Studies and Reports·Silvina Angeles-HernándezPedro Mendoza-de-Gives
Jul 25, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Aya C TakiRobin B Gasser

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.