Biobased monoliths for adenovirus purification

ACS Applied Materials & Interfaces
Cláudia S M FernandesA Cecília A Roque

Abstract

Adenoviruses are important platforms for vaccine development and vectors for gene therapy, increasing the demand for high titers of purified viral preparations. Monoliths are macroporous supports regarded as ideal for the purification of macromolecular complexes, including viral particles. Although common monoliths are based on synthetic polymers as methacrylates, we explored the potential of biopolymers processed by clean technologies to produce monoliths for adenovirus purification. Such an approach enables the development of disposable and biodegradable matrices for bioprocessing. A total of 20 monoliths were produced from different biopolymers (chitosan, agarose, and dextran), employing two distinct temperatures during the freezing process (-20 °C and -80 °C). The morphological and physical properties of the structures were thoroughly characterized. The monoliths presenting higher robustness and permeability rates were further analyzed for the nonspecific binding of Adenovirus serotype 5 (Ad5) preparations. The matrices presenting lower nonspecific Ad5 binding were further functionalized with quaternary amine anion-exchange ligand glycidyltrimethylammonium chloride hydrochloride by two distinct methods, and their performanc...Continue Reading

References

May 27, 2003·BioTechniques·Kaoru YamadaChristopher E Walsh
Nov 15, 2003·Biomaterials·Fergal J O'BrienLorna Gibson
Feb 24, 2004·The Journal of Gene Medicine·Christoph Volpers, Stefan Kochanek
Jul 6, 2004·Journal of Chromatography. a·Per-Erik GustavssonPer-Olof Larsson
Sep 10, 2004·Journal of Separation Science·Alois Jungbauer, Rainer Hahn
Sep 29, 2004·Molecular Therapy : the Journal of the American Society of Gene Therapy·Nia Tatsis, Hildegund C J Ertl
Mar 24, 2005·Journal of Chromatography. a·Alois Jungbauer
Feb 5, 2008·Journal of Chromatography. a·Alois Jungbauer, Rainer Hahn
Oct 22, 2008·Journal of Chromatography. a·Mark R Etzel, William T Riordan
Feb 6, 2009·Biotechnology Progress·Cristina PeixotoPaula M Alves
Feb 12, 2009·Analytical and Bioanalytical Chemistry·Anna Nordborg, Emily F Hilder
May 21, 2009·Journal of Biomaterials Science. Polymer Edition·Era Jain, Ashok Kumar
Mar 2, 2010·Journal of Virological Methods·Petra KrambergerMatjaz Peterka
Mar 29, 2011·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Dubravko ForcicAleš Strancar
Jul 6, 2012·Gene Therapy·P FernandesP M Alves
Oct 24, 2012·Virology·Hanna M OksanenDennis H Bamford
Jan 29, 2013·The Journal of Gene Medicine·Samantha L GinnJo Wixon
Mar 8, 2013·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Aleš PodgornikNika Lendero Krajnc
May 8, 2013·Biotechnology Journal·Telma BarrosoAna Aguiar-Ricardo
May 13, 2014·Journal of Chromatography. a·Piergiuseppe NestolaJosé P B Mota
Jun 7, 2013·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Timothy M A HendersonAndrea J O'Connor

❮ Previous
Next ❯

Citations

Apr 26, 2020·Biotechnology Journal·Ana Cecília Afonso RoqueMarcel Ottens
Sep 15, 2019·Analytica Chimica Acta·María Vergara-BarberánJosé Manuel Herrero-Martínez
Jun 16, 2021·Journal of Separation Science·Renan VitekJorge C Masini

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Dubravko ForcicAleš Strancar
Molecular Therapy : the Journal of the American Society of Gene Therapy
Jayanta Roy-Chowdhury, Marshall S Horwitz
© 2022 Meta ULC. All rights reserved