Biocatalytic carbon paste sensors based on a mediator pasting liquid

Analytical Chemistry
Nathan S LawrenceJoseph Wang

Abstract

The preparation and advantages of a new generation of carbon paste enzyme electrodes where the redox mediator acts also as the pasting liquid are described. The mediator pasting liquid concept is illustrated for amperometric biosensing of glucose in connection with either the tert-pentylferrocene or n-butylferrocene mediator/binder along with the glucose oxidase enzyme. The attractive performance and advantages of the new device is indicated from comparison to a conventional carbon paste biosensor using a mineral oil binder and the dimethylferrocene electron acceptor. The simplified preparation of the biosensor is coupled with a greatly improved sensitivity and an extended linear range. The mediator pasting liquid imparts high thermal stability onto the embedded enzyme and leads to good resistance to oxygen effects. Owing to the huge mediator reservoir, stability problems associated with the leaching of the mediator are greatly reduced. The fundamental aspects of the electrode behavior have been examined first in the absence of the enzyme. Variables affecting the performance of the new carbon paste biosensor have been investigated and optimized. Such use of the electron acceptor as a binder as well as the mediator offers consid...Continue Reading

References

Apr 1, 1984·Analytical Chemistry·A E CassA P Turner

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Citations

Jun 15, 2006·Analytical Chemistry·Eric Bakker, Yu Qin
May 15, 2007·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·M R Nateghi, M H Fallahian
Apr 12, 2008·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Atsunori HiratsukaHitoshi Muguruma
Aug 13, 2010·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Liqiang LuoDongmei Deng
Dec 20, 2005·Analytical Biochemistry·Sanket TembeStanislaus F D'Souza
Nov 1, 2005·Biosensors & Bioelectronics·Atsunori HiratsukaIsao Karube
May 7, 2016·International Journal of Biological Macromolecules·Elham HeydariZahra Bagheryan
Jan 24, 2006·Chemistry : a European Journal·Bikash Kumar Jena, C Retna Raj
Sep 9, 2010·ACS Applied Materials & Interfaces·Ta-Ming ChengHsin-Tien Chiu
Mar 30, 2007·Organic Letters·Tianzhi Zhang, Eric V Anslyn
May 10, 2008·Chemical Reviews·Adam Heller, Ben Feldman

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