Biocatalytic Degradation Efficiency of Postconsumer Polyethylene Terephthalate Packaging Determined by Their Polymer Microstructures

Advanced Science
Ren WeiWolfgang Zimmermann

Abstract

Polyethylene terephthalate (PET) is the most important mass-produced thermoplastic polyester used as a packaging material. Recently, thermophilic polyester hydrolases such as TfCut2 from Thermobifida fusca have emerged as promising biocatalysts for an eco-friendly PET recycling process. In this study, postconsumer PET food packaging containers are treated with TfCut2 and show weight losses of more than 50% after 96 h of incubation at 70 °C. Differential scanning calorimetry analysis indicates that the high linear degradation rates observed in the first 72 h of incubation is due to the high hydrolysis susceptibility of the mobile amorphous fraction (MAF) of PET. The physical aging process of PET occurring at 70 °C is shown to gradually convert MAF to polymer microstructures with limited accessibility to enzymatic hydrolysis. Analysis of the chain-length distribution of degraded PET by nuclear magnetic resonance spectroscopy reveals that MAF is rapidly hydrolyzed via a combinatorial exo- and endo-type degradation mechanism whereas the remaining PET microstructures are slowly degraded only by endo-type chain scission causing no detectable weight loss. Hence, efficient thermostable biocatalysts are required to overcome the competit...Continue Reading

References

Jan 5, 1979·Molecular & General Genetics : MGG·S Chang, S N Cohen
Mar 14, 2001·Journal of Biotechnology·R J MüllerW D Deckwer
Jan 1, 2004·Environmental Science and Pollution Research International·Daniela CartaClaudio D'Angeli
Aug 24, 2005·Journal of Biotechnology·M A M E VertommenM M C G Warmoeskerken
May 1, 1961·Journal of Bacteriology·C Anagnostopoulos, J Spizizen
Sep 2, 2006·Biotechnology and Bioengineering·Yang YangWolf-Dieter Deckwer
Feb 15, 2013·Nature·Chelsea M RochmanRichard C Thompson
Apr 15, 2014·Applied Microbiology and Biotechnology·Christian RothNorbert Sträter
Aug 19, 2014·Advances in Applied Microbiology·Ren WeiWolfgang Zimmermann
Feb 14, 2015·Science·Jenna R JambeckKara Lavender Law
Mar 12, 2016·Science·Shosuke YoshidaKohei Oda
Mar 31, 2016·Microbial Cell Factories·Tina N PlossJan Maarten van Dijl
Apr 4, 2017·Microbial Biotechnology·Ren Wei, Wolfgang Zimmermann
Apr 13, 2017·Microbial Biotechnology·Ren Wei, Wolfgang Zimmermann
Jun 3, 2017·Microbial Biotechnology·Felice QuartinelloGeorg M Guebitz
Jul 18, 2017·Microbial Biotechnology·Tanja Narancic, Kevin E O'Connor
Aug 5, 2017·Science Advances·Roland GeyerKara Lavender Law
Apr 19, 2018·Proceedings of the National Academy of Sciences of the United States of America·Harry P AustinGregg T Beckham

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Citations

Oct 12, 2019·Microbial Cell Factories·Daniel MoogUwe G Maier
May 7, 2020·Frontiers in Microbiology·Jiakang RuYu Yang
Mar 3, 2020·Frontiers in Microbiology·Alexander BollingerKarl-Erich Jaeger
Jul 7, 2020·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Wolfgang Zimmermann
May 16, 2020·Frontiers in Microbiology·Patricia FalkensteinChen Song
Dec 18, 2020·Frontiers in Bioengineering and Biotechnology·Ankita MauryaSunil Kumar Khare
Jan 13, 2021·Environmental Sciences Europe·Cristina Palacios-MateoGunnar Seide
Mar 2, 2021·Frontiers in Microbiology·Ren Wei, Nick Wierckx
Jan 21, 2021·Chembiochem : a European Journal of Chemical Biology·Alessandro BerselliMaria Cristina Menziani
Mar 24, 2021·International Journal of Biological Macromolecules·Xiangxi MengJian Tian
Apr 11, 2021·Journal of Hazardous Materials·Wenlong YueNing-Yi Zhou
Apr 19, 2021·Metabolic Engineering·Till TisoLars M Blank
May 26, 2021·The Science of the Total Environment·Sameh S AliJianzhong Sun
Jun 3, 2021·International Journal of Molecular Sciences·Nikolina AtanasovaMargarita Kambourova
Jun 15, 2021·World Journal of Microbiology & Biotechnology·Julio Cesar Soares SalesMaria Alice Zarur Coelho
Jun 15, 2021·Frontiers in Bioengineering and Biotechnology·Lakshika Dissanayake, Lahiru N Jayakody
Jul 10, 2021·Advanced Materials·Xin ZhaoHan Sen Soo
Jul 10, 2021·Frontiers in Bioengineering and Biotechnology·Efstratios NikolaivitsJasmina Nikodinovic-Runic
Nov 11, 2021·Biotechnology and Bioengineering·Jenny Arnling BååthDoris Ribitsch

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Methods Mentioned

BETA
nuclear
scanning electron microscopy
size exclusion chromatography
chips
circular dichroism
chip
differential scanning calorimetry
HiC
nuclear magnetic resonance
NMR

Software Mentioned

MestReNova

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