Biocatalytic production of adipic acid from glucose using engineered Saccharomyces cerevisiae

Metabolic Engineering Communications
Kaushik RajRadhakrishnan Mahadevan

Abstract

Adipic acid is an important industrial chemical used in the synthesis of nylon-6,6. The commercial synthesis of adipic acid uses petroleum-derived benzene and releases significant quantities of greenhouse gases. Biocatalytic production of adipic acid from renewable feedstocks could potentially reduce the environmental damage and eliminate the need for fossil fuel precursors. Recently, we have demonstrated the first enzymatic hydrogenation of muconic acid to adipic acid using microbial enoate reductases (ERs) - complex iron-sulfur and flavin containing enzymes. In this work, we successfully expressed the Bacillus coagulans ER in a Saccharomyces cerevisiae strain producing muconic acid and developed a three-stage fermentation process enabling the synthesis of adipic acid from glucose. The ability to express active ERs and significant acid tolerance of S. cerevisiae highlight the applicability of the developed yeast strain for the biocatalytic production of adipic acid from renewable feedstocks.

Citations

Sep 14, 2018·World Journal of Microbiology & Biotechnology·Bonnie A McNeil, David T Stuart
Sep 25, 2019·Bioprocess and Biosystems Engineering·Jung-Hyun JuWon-Kyung Hong
Sep 27, 2018·Biotechnology for Biofuels·Si-Yu LiYun-Peng Chao
Feb 28, 2019·Microbial Cell Factories·Damla HuccetogullariSang Yup Lee
Oct 20, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jacob SagManfred Döring
Jul 24, 2018·Engineering in Life Sciences·Jia-Le YuJian-Jiang Zhong
Mar 28, 2020·Applied Microbiology and Biotechnology·Xi ZhangYu Deng
Aug 10, 2020·Scientific Reports·Nicholas S KruyerPamela Peralta-Yahya
Oct 24, 2020·The Journal of Microbiology·Xi ZhangYu Deng
Jan 8, 2021·Natural Product Reports·Yang LiXiulai Chen
Oct 20, 2019·Journal of Biotechnology·Neil Butler, Aditya M Kunjapur
Mar 13, 2020·Biotechnology Journal·Yu Jung SohnSi Jae Park
Aug 18, 2020·The Journal of Nutritional Biochemistry·Zuzana RacovaPavel Anzenbacher
Dec 20, 2020·Trends in Biotechnology·Alessandra Biz, Radhakrishnan Mahadevan
Feb 11, 2021·Environmental Science and Pollution Research International·Ana AriasMaria Teresa Moreira
Aug 14, 2020·ACS Synthetic Biology·Jack T SuitorStephen Wallace
Aug 23, 2020·Metabolic Engineering·Kaushik RajRadhakrishnan Mahadevan

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BETA
biosensor
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