PMID: 22332367Feb 16, 2012Paper

Biocatalytic synthesis of pharmacology perspective stigmast-4-en-3-one using Rhodococci cells

Bioorganicheskaia khimiia
E M NogovitsinaI B Ivshina

Abstract

The conditions for a directed biocatalytic oxidation of beta-sitosterol to a pharmacologically promising stigmast-4-en-3-one using Rhodococcus actinobacteria were selected. It was shown that palmitic acid induced the cholesterol oxidase reaction and allowed for the decrease in the bioconversion process duration from 7 to 5 days. The maximum level ofstigmast-4-ene-3-one formation was achieved using n-hexadecane as an additional growth substrate. With increased concentrations of beta-sitosterol (up to 2 g/L) an effective target product formation (80%) was achieved in the presence of Tween-80 and beta-cyclodextrine. R. erythropolis strains were 1.5-2 times more active than R. ruber strains in catalyzing the beta-sitosterol biotransformation process.

References

Nov 21, 2007·Applied Biochemistry and Biotechnology·Wenyu LuBing Sun
Mar 11, 2008·Bioresource Technology·Alok Malaviya, James Gomes
Jun 6, 2009·Applied Microbiology and Biotechnology·Noriyuki Doukyu
Oct 22, 2009·The FEBS Journal·Loredano PollegioniGianluca Molla

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