PMID: 6300658Feb 1, 1983

Biochemical activities of T-antigen proteins encoded by simian virus 40 A gene deletion mutants

Molecular and Cellular Biology
R ClarkR Tjian

Abstract

We have analyzed T antigens produced by a set of simian virus 40 (SV40) A gene deletion mutants for ATPase activity and for binding to the SV40 origin of DNA replication. Virus stocks of nonviable SV40 A gene deletion mutants were established in SV40-transformed monkey COS cells. Mutant T antigens were produced in mutant virus-infected CV1 cells. The structures of the mutant T antigens were characterized by immunoprecipitation with monoclonal antibodies directed against distinct regions of the T-antigen molecule. T antigens in crude extracts prepared from cells infected with 10 different mutants were immobilized on polyacrylamide beads with monoclonal antibodies, quantified by Coomassie blue staining, and then assayed directly for T antigen-specific ATPase activity and for binding to the SV40 origin of DNA replication. Our results indicate that the T antigen coding sequences required for origin binding map between 0.54 and 0.35 map units on the SV40 genome. In contrast, sequences closer to the C terminus of T antigen (between 0.24 and 0.20 map units) are required for ATPase activity. The presence of the ATPase activity correlated closely with the ability of the mutant viruses to replicate and to transform nonpermissive cells. T...Continue Reading

Citations

Sep 1, 1986·Virology·L Sompayrac, K J Danna
Apr 21, 2001·Virology·K F Sachsenmeier, James M Pipas
Dec 17, 2008·Virology·James M Pipas
Aug 31, 2000·Annual Review of Biochemistry·S S Patel, K M Picha
Sep 1, 1989·European Journal of Biochemistry·H KienzleR Knippers
Jan 1, 1997·Critical Reviews in Biochemistry and Molecular Biology·Peter A Bullock
Dec 15, 1987·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·T J KellyM S Wold
Dec 15, 1987·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M P FairmanB Stillman
Jun 23, 1995·The Journal of Biological Chemistry·B S Collins, James M Pipas
May 28, 2005·Journal of Virology·Jennifer A MarkovicsJames M Pipas
Jun 29, 2007·Journal of Virology·Amélie Fradet-TurcotteJacques Archambault

Related Concepts

DNA-dependent ATPase
Antigens, Viral
Antigens, Neoplasm, Viral
Partial Monosomy
DNA, Viral
Genes, Viral
Simian virus 40
Virus Replication

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