Biochemical and biological activities of the venom of the Chinese pitviper Zhaoermia mangshanensis, with the complete amino acid sequence and phylogenetic analysis of a novel Arg49 phospholipase A2 myotoxin

Toxicon : Official Journal of the International Society on Toxinology
Dietrich MebsLourival D Possani

Abstract

Zhaoermia mangshanensis (formerly Trimeresurus mangshanensis, Ermia mangshanensis) represents a monotypic genus of pitviper known only from Mt Mang in China's Hunan Province, and is among the largest and most spectacular of Asian venomous snakes. The venom of Zhaoermia exhibits high coagulant activity on bovine and human fibrinogen and human plasma, high phosphodiesterase and arginine ester hydrolytic activity, and moderate to low l-amino acid oxidase, kallikrein, caseinolytic, phospholipase A(2) (PLA(2)), haemorrhagic and myotoxic activities. The approximate i.p. LD(50) of the venom in mice was estimated to be 4 mg/kg. We purified the major toxin of Zhaoermia venom by gel-filtration, cation-exchange chromatography and HPLC. The toxin, a homodimer with an experimental monomeric mass of 13,972 Da, induced edema and myonecrosis in mice, but was devoid of detectable PLA(2) catalytic activity. Its complete amino acid sequence is composed of 121 amino acid residues cross-linked by seven disulfide bridges, and shows more than 80% identity to two Lys49-PLA(2)s from distantly related Asian pitvipers, Protobothrops mucrosquamatus and Calloselasma rhodostoma. Phylogenetic analysis of the novel toxin, zhaoermiatoxin, confirmed that it is ...Continue Reading

References

Feb 1, 1991·Archives of Biochemistry and Biophysics·B FrancisI I Kaiser
Jan 1, 1990·Pharmacology & Therapeutics·D Mebs, C L Ownby
May 1, 1990·Archives of Biochemistry and Biophysics·I I KaiserG V Odell
Jan 1, 1989·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·N H TanC S Tan
Nov 30, 1989·Biochimica Et Biophysica Acta·I KrizajF Gubensek
Oct 1, 1989·International Journal of Peptide and Protein Research·R M Kini, H J Evans
Jan 1, 1989·Toxicon : Official Journal of the International Society on Toxinology·I Claus, D Mebs
Jan 1, 1988·Toxicon : Official Journal of the International Society on Toxinology·M I Homsi-BrandeburgoJ R Giglio
Apr 29, 1985·Biochimica Et Biophysica Acta·A Ritonja, F Gubensek
Apr 1, 1971·Toxicon : Official Journal of the International Society on Toxinology·W F KocholatyT A Billings
Jun 1, 1965·Biochimica Et Biophysica Acta·G V Marinetti
Mar 1, 1995·Toxicon : Official Journal of the International Society on Toxinology·R K ArniJ R Giglio
May 1, 1995·Toxicon : Official Journal of the International Society on Toxinology·L C MancusoJ R Giglio
Jun 6, 1995·Proceedings of the National Academy of Sciences of the United States of America·K NakashimaS Hattori
Feb 1, 1993·Journal of Protein Chemistry·A C CintraJ R Giglio
Mar 1, 1993·Toxicon : Official Journal of the International Society on Toxinology·E K Johnson, C L Ownby
Dec 1, 1995·Journal of Molecular Evolution·T OgawaM Ohno
Nov 1, 1995·Toxicon : Official Journal of the International Society on Toxinology·J M Gutiérrez, B Lomonte
Aug 1, 1996·Toxicon : Official Journal of the International Society on Toxinology·R K Arni, R J Ward
Sep 1, 1997·Nucleic Acids Research·S F AltschulD J Lipman
May 30, 1998·Toxicon : Official Journal of the International Society on Toxinology·E CarredanoH Eklund
May 30, 1998·Toxicon : Official Journal of the International Society on Toxinology·Y ZhangW Y Wang
Aug 5, 1998·Toxicon : Official Journal of the International Society on Toxinology·H PanX F Wu
Jun 21, 2001·Biochemical and Biophysical Research Communications·A H de OliveiraR J Ward
Aug 29, 2001·Bioinformatics·J P Huelsenbeck, F Ronquist
Dec 18, 2001·Science·J P HuelsenbeckJ P Bollback
Feb 5, 2003·Toxicon : Official Journal of the International Society on Toxinology·Dietrich MebsThomas Ziegler
Apr 3, 2003·Nature Reviews. Genetics·Mark Holder, Paul O Lewis
Aug 13, 2003·Bioinformatics·Fredrik Ronquist, John P Huelsenbeck

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Citations

Mar 20, 2013·BioMed Research International·Ji-Fu WeiShaoheng He
Jul 11, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Mário T MurakamiRaghuvir K Arni
Jun 5, 2012·Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia·Jiri ValentaMichal Otahal
Feb 3, 2016·Clinical Toxicology : the Official Journal of the American Academy of Clinical Toxicology and European Association of Poisons Centres and Clinical Toxicologists·Travis D OlivesJon B Cole
Feb 26, 2016·Toxicon : Official Journal of the International Society on Toxinology·Inn-Ho TsaiKai-Fa Huang
Jun 12, 2010·Toxicon : Official Journal of the International Society on Toxinology·Ji-Fu WeiShao-Heng He
Dec 29, 2009·Toxicon : Official Journal of the International Society on Toxinology·Ji-Fu WeiShao-Heng He
Oct 31, 2009·Toxicon : Official Journal of the International Society on Toxinology·M Gallacci, W L G Cavalcante
Sep 12, 2015·Toxicon : Official Journal of the International Society on Toxinology·Anita MalhotraRoger S Thorpe
Jun 28, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Débora C O NunesVeridiana M Rodrigues
Jul 9, 2013·Toxicon : Official Journal of the International Society on Toxinology·Anita MalhotraRoger S Thorpe
Mar 28, 2017·The Journal of Venomous Animals and Toxins Including Tropical Diseases·Norival A Santos-Filho, Claudia T Santos

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