Biochemical and biological characterization of a new oxidized avidin with enhanced tissue binding properties.

The Journal of Biological Chemistry
Antonio VerdolivaR De Santis

Abstract

Chicken avidin and bacterial streptavidin are widely employed in vitro for their capacity to bind biotin, but their pharmacokinetics and immunological properties are not always optimal, thereby limiting their use in medical treatments. Here we investigate the biochemical and biological properties of a new modified avidin, obtained by ligand-assisted sodium periodate oxidation of avidin. This method allows protection of biotin-binding sites of avidin from inactivation caused by the oxidation step and delay of avidin clearance from injected tissue by generation of aldehyde groups from avidin carbohydrate moieties. Oxidized avidin shows spectroscopic properties similar to that of native avidin, indicating that tryptophan residues are spared from oxidation damage. In strict agreement with these results, circular dichroism and isothermal titration calorimetry analyses confirm that the ligand-assisted oxidation preserves the avidin protein structure and its biotin binding capacity. In vitro cell binding and in vivo tissue residence experiments demonstrate that aldehyde groups provide oxidized avidin the property to bind cellular and interstitial protein amino groups through Schiff's base formation, resulting in a tissue half-life of ...Continue Reading

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Citations

Apr 29, 2010·Cancer Biotherapy & Radiopharmaceuticals·Rita De SantisPaolo Carminati
Sep 25, 2010·Cancer Biotherapy & Radiopharmaceuticals·Fiorella PetronzelliRita De Santis
Jun 28, 2011·PloS One·Rita De SantisCarlo Antonio Nuzzolo
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Mar 24, 2011·Basic & Clinical Pharmacology & Toxicology·Fiorella PetronzelliRita De Santis
Sep 12, 2015·The Journal of Organic Chemistry·Osamu HayashidaShuhei Kusano
Jul 15, 2015·Cancer Biotherapy & Radiopharmaceuticals·Claudio AlbertoniRita De Santis
Jun 18, 2017·Angewandte Chemie·Vincent EscandePaul T Anastas
Oct 11, 2013·Organic & Biomolecular Chemistry·Anne I GermerothAlison N Hulme
Nov 24, 2017·Analytical and Bioanalytical Chemistry·Giuseppe GianniniRita De Santis
Apr 18, 2012·International Journal of Immunopathology and Pharmacology·E NuceraR De Santis
Aug 29, 2020·Pharmaceutics·Alberto JuanCarlos Alonso-Moreno

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