Biochemical and Cellular Assessment of Acetabular Chondral Flaps Identified During Hip Arthroscopy

Arthroscopy : the Journal of Arthroscopic & Related Surgery : Official Publication of the Arthroscopy Association of North America and the International Arthroscopy Association
Sanaz HaririMarc R Safran

Abstract

To analyze chondral flaps debrided during hip arthroscopy to determine their biochemical and cellular composition. Thirty-one full-thickness acetabular chondral flaps were collected during hip arthroscopy. Biochemical analysis was undertaken in 21 flaps from 20 patients, and cellular viability was determined in 10 flaps from 10 patients. Biochemical analysis included concentrations of (1) DNA (an indicator of chondrocyte content), (2) hydroxyproline (an indicator of collagen content), and (3) glycosaminoglycan (an indicator of chondrocyte biosynthesis). Higher values for these parameters indicated more healthy tissue. The flaps were examined to determine the percentage of viable chondrocytes. The percentage of acetabular chondral flap specimens that had concentrations within 1 SD of the mean values reported in previous normal cartilage studies was 38% for DNA, 0% for glycosaminoglycan, and 43% for hydroxyproline. The average cellular viability of our acetabular chondral flap specimens was 39% (SD, 14%). Only 2 of the 10 specimens had more than half the cells still viable. There was no correlation between (1) the gross examination of the joint or knowledge of the patient's demographic characteristics and symptoms and (2) biochem...Continue Reading

References

Jan 1, 1992·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·R L SmithM C van der Meulen
Oct 1, 1988·Analytical Biochemistry·Y J KimA J Grodzinsky
Aug 1, 1971·The Journal of Clinical Investigation·H J Mankin, L Lippiello
Feb 1, 1970·Clinica Chimica Acta; International Journal of Clinical Chemistry·I Bergman, R Loxley
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
Jul 13, 2005·The American Journal of Sports Medicine·R Todd AllenDavid Amiel
Apr 6, 2006·The Journal of Bone and Joint Surgery. American Volume·Theodore MalininBill E Buck
May 18, 2010·Sports Medicine and Arthroscopy Review·Yi-Meng Yen, Mininder S Kocher
Mar 11, 2011·The American Journal of Sports Medicine·Michael S LinnDavid Amiel

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Citations

Sep 5, 2017·Orthopaedic Journal of Sports Medicine·Francisco Rodriguez-FontanCecilia Pascual-Garrido
Jul 6, 2018·Hip International : the Journal of Clinical and Experimental Research on Hip Pathology and Therapy·Pascal C HaefeliLorenz Büchler
Oct 13, 2017·The American Journal of Sports Medicine·Vonda J WrightJohnny Huard
Oct 28, 2020·The American Journal of Sports Medicine·Miranda J RogersTravis G Maak

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