PMID: 7523597Nov 1, 1994Paper

Biochemical and cellular properties of three immortalized Schwann cell lines expressing different levels of the myelin-associated glycoprotein

Journal of Neurochemistry
K TodaR H Quarles

Abstract

Biochemical and cellular properties of three immortalized Schwann cell lines expressing different levels of the myelin-associated glycoprotein (MAG) were compared. The S16 line generated by repetitive passaging was described previously and expresses a level of MAG comparable to that in adult sciatic nerve. The S42 line was generated independently by the same procedure, divides more slowly than the S16 line, and expresses an even higher level of MAG. The S16Y line arose spontaneously from a passage of the S16 cells, divides much more rapidly, and does not express MAG. The levels of MAG expression in the three lines are inversely related to their rates of proliferation, and MAG mRNA levels parallel the amounts of MAG. The S16 and S42 lines consist mainly of flat cells at low density and develop many processes at high density, whereas most of the S16Y cells are spindle-shaped, resembling primary Schwann cells in appearance. Surface immunostaining with the O4 antibody was positive for the S16 and S42 cells and negative for the S16Y cells, but all three lines were negative for surface staining with the O1 antibody. The overall protein compositions of the three lines are very similar, but the S16 and S42 cells express larger amounts ...Continue Reading

Citations

Nov 7, 2006·Neuropathology and Applied Neurobiology·C O HanemannT Utermark
Aug 23, 2006·Journal of Neurochemistry·Sung-Wook JangJohn Svaren
Dec 4, 2010·Neurochemical Research·George H De Vries, Anne I Boullerne
Jun 9, 2016·Nature Neuroscience·Yannick PoitelonMaria Laura Feltri
Feb 28, 2007·Molecular and Cellular Biology·Scott E LeBlancJohn Svaren
Dec 24, 2010·PloS One·Anthony AntonellisUNKNOWN NISC Comparative Sequencing Program
Feb 23, 2020·Journal of Tissue Engineering and Regenerative Medicine·Tanya SinghMaribel Vazquez
Sep 5, 2002·Journal of Neuroscience Research·Mehreen HaiPragna I Patel
Aug 5, 2015·Journal of Cellular Biochemistry·Isis C SrokaAnne E Cress
Jun 14, 2016·Nature Neuroscience·Susanne QuintesKlaus-Armin Nave
Apr 12, 2012·Nucleic Acids Research·Rajini SrinivasanJohn Svaren
Aug 27, 2015·The European Journal of Neuroscience·S GeunaC Grothe
Jun 12, 2016·Human Molecular Genetics·Camila Lopez-AnidoJohn Svaren
Aug 11, 2020·BMC Genomics·Elizabeth A FogartyAnthony Antonellis
Dec 18, 2003·Annals of the New York Academy of Sciences·Dusanka S SkundricPhilip Mataverde
Dec 14, 2019·Annals of Clinical and Translational Neurology·Hongge WangMichael E Shy
Nov 29, 2016·Journal of Neurochemistry·José F Rodríguez-MolinaJohn Svaren
Dec 5, 2020·Journal of Tissue Engineering and Regenerative Medicine·Richard N CliverMaribel Vazquez
Dec 24, 2005·The Journal of Biological Chemistry·Scott E LeBlancJohn Svaren
May 6, 2008·The Journal of Biological Chemistry·Gennifer M MagerJohn Svaren
May 2, 2003·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Kazuhiko WatabeNorie Araki
Feb 21, 2018·Neuroscience·Melissa R Wrobel, Harini G Sundararaghavan
Jul 19, 2015·Journal of Cell Science·Eva Sonnenberg-RiethmacherDieter Riethmacher
Jun 26, 1998·Progress in Lipid Research·I Ishizuka
Jun 16, 2011·BMC Developmental Biology·Megana K PrasadAndrew S McCallion

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Adhesion Molecules in the Brain

Cell adhesion molecules found on cell surface help cells bind with other cells or the extracellular matrix to maintain structure and function. Here is the latest research on their role in the brain.