Biochemical and Expression Analyses of the Rice Cinnamoyl-CoA Reductase Gene Family

Frontiers in Plant Science
Hye Lin ParkMan-Ho Cho

Abstract

Cinnamoyl-CoA reductase (CCR) is the first committed enzyme in the monolignol pathway for lignin biosynthesis and catalyzes the conversion of hydroxycinnamoyl-CoAs into hydroxycinnamaldehydes. In the rice genome, 33 genes are annotated as CCR and CCR-like genes, collectively called OsCCRs. To elucidate the functions of OsCCRs, their phylogenetic relationships, expression patterns at the transcription levels and biochemical characteristics were thoroughly analyzed. Of the 33 OsCCRs, 24 of them encoded polypeptides of lengths similar to those of previously identified plant CCRs. The other nine OsCCRs had much shorter peptide lengths. Phylogenetic tree and sequence similarities suggested OsCCR4, 5, 17, 18, 19, 20, and 21 as likely candidates for functional CCRs in rice. To elucidate biochemical functions, OsCCR1, 5, 17, 19, 20, 21, and 26 were heterologously expressed in Escherichia coli and the resulting recombinant OsCCRs were purified to apparent homogeneity. Activity assays of the recombinant OsCCRs with hydroxycinnamoyl-CoAs revealed that OsCCR17, 19, 20, and 21 were biochemically active CCRs, in which the NAD(P)-binding and NADP-specificity motifs as well as the CCR signature motif were fully conserved. The kinetic parameter...Continue Reading

References

Jun 26, 2001·Phytochemistry·L A Donaldson
Mar 7, 2002·Analytical Biochemistry·Till Beuerle, Eran Pichersky
Apr 16, 2005·Current Opinion in Biotechnology·Basia Vinocur, Arie Altman
May 5, 2005·Plant & Cell Physiology·Laigeng LiVincent L Chiang
Jul 12, 2005·Biological Chemistry·Qing-Hu Ma, Bin Tian
Aug 5, 2005·Annual Review of Phytopathology·Richard N Strange, Peter R Scott
Aug 27, 2005·Plant Physiology and Biochemistry : PPB·M BaltasJ Grima-Pettenati
Dec 29, 2005·Proceedings of the National Academy of Sciences of the United States of America·Tsutomu KawasakiKo Shimamoto
May 13, 2006·Biochemical and Biophysical Research Communications·Mukesh JainJitendra P Khurana
Oct 29, 1982·Science·J S Boyer
Feb 13, 2008·The Plant Journal : for Cell and Molecular Biology·Atsushi IshiharaKyo Wakasa
Jan 1, 2008·Advances in Bioinformatics·Tomas HruzPhilip Zimmermann
Apr 10, 2010·Journal of Integrative Plant Biology·Jullyana Cristina Magalhães Silva MouraPaulo Mazzafera
May 18, 2010·Plant Physiology·Ruben VanholmeWout Boerjan
Sep 1, 2002·Molecular Plant Pathology·Richard A DixonLiangjiang Wang
Sep 3, 2010·Annual Review of Genetics·Nicholas D Bonawitz, Clint Chapple
Jul 19, 2011·Plant Science : an International Journal of Experimental Plant Biology·Abdelali BarakatJohn E Carlson
Jan 3, 2012·Trends in Plant Science·Ishita AhujaAtle M Bones
Aug 28, 2012·Plant Science : an International Journal of Experimental Plant Biology·Dominik K GroßkinskyThomas Roitsch
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar

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Citations

Jun 30, 2019·Plant Molecular Biology·Sávio Siqueira FerreiraIgor Cesarino
Oct 20, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hye Lin ParkMan-Ho Cho
Mar 4, 2020·Molecular Genetics and Genomics : MGG·Douglas Jardim-MessederGilberto Sachetto-Martins
Jan 7, 2021·Functional & Integrative Genomics·Douglas Jardim-MessederGilberto Sachetto-Martins
Jan 14, 2020·Plant Science : an International Journal of Experimental Plant Biology·Lina Gomez-CanoJohn Gray

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Datasets Mentioned

BETA
ABE01883
AY771357

Methods Mentioned

BETA
PCR
electrophoresis
salt treatment
GTPase

Software Mentioned

SPSS
MEGA
Clustal
Multi Experiment Viewer

Related Concepts