Biochemical and functional characterization of Epstein-Barr virus-encoded BARF1 protein: interaction with human hTid1 protein facilitates its maturation and secretion

Oncogene
L WangD X Liu

Abstract

EBV BARF1 gene encodes a secretory protein with transforming and mitogenic activities. In this report, the post-translational modification, folding, maturation and secretion of BARF1 are systematically studied by site-directed mutagenesis and overexpression of the protein in mammalian cells using the vaccinia/T7 system. The protein was shown to be post-translationally modified by N-linked glycosylation on the asparagine 95 residue. This modification was confirmed to be essential for the maturation and secretion of the protein. Analysis of the four cysteine residues by site-directed mutagenesis demonstrated that cysteine 146 and 201 were essential for proper folding and secretion of the protein. To search for human proteins involved in the maturation process of the protein, a yeast two-hybrid screening was carried out using the BARF1 sequence from amino acids 21-221 (BARF1Delta) as bait, leading to the identification of human hTid1 protein as a potential interacting protein. This interaction was subsequently confirmed by coimmunoprecipitation and dual immunofluorescent labeling of cells coexpressing BARF1 and hTid1, and the interaction domain in hTid1 was mapped to amino acids 149-320. Interestingly, coexpression of BARF1 with h...Continue Reading

References

Aug 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·P BorkA Valencia
Feb 15, 1994·International Journal of Cancer. Journal International Du Cancer·M BouzidT Ooka
May 19, 1998·Cell Stress & Chaperones·M E Cheetham, A J Caplan
Jul 21, 1999·Proceedings of the National Academy of Sciences of the United States of America·J SykenK Münger
Oct 27, 2001·The Journal of Biological Chemistry·S SarkarS Pestka
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Chi-Yong Eom, I Robert Lehman
Apr 3, 2002·The Journal of Biological Chemistry·Hua ChengCecilia Cheng-Mayer
Apr 6, 2004·Oncogene·Alhousseynou SallTadamasa Ooka
Jul 10, 2004·Reviews of Physiology, Biochemistry and Pharmacology·M P Mayer
Sep 15, 2004·Developmental Cell·Cheng-Chun WangWanjin Hong
Nov 18, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Christian P SchaafJohannes W G Janssen

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Citations

Jun 6, 2008·Virology Journal·Sylvie Fiorini, Tadamasa Ooka
Sep 3, 2013·Reviews in Medical Virology·Eveline K HoebeJaap M Middeldorp
Oct 2, 2007·Virus Research·Minghui FuXunzhang Wang
Sep 18, 2007·The Journal of General Virology·Mireille de Turenne-Tessier, Tadamasa Ooka
Oct 24, 2020·Current Genomics·Mst Shamima KhatunHiroyuki Kurata

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