Biochemical and functional characterization of basal lamina-bound agrin in the chick central nervous system

The European Journal of Neuroscience
S Kröger, S Mann

Abstract

Agrin is a high-molecular weight extracellular matrix molecule, initially purified from the electric organ of the marine ray Torpedo californica, which induces on the surface of cultured myotubes the formation of postsynaptic specializations similar to those found at the neuromuscular junction. Agrin immunoreactivity is highly concentrated in the basal lamina of the synaptic cleft but is also found in a number of other tissues where its function is not known. We characterized agrin associated with two basal laminae from the central nervous system, the inner limiting membrane of the retina and the mesencephalic external limiting membrane. A major broad band with an apparent molecular weight of > 300 kDa was identified in immunoblots of isolated basal laminae from retina, mesencephalon, kidney and muscle, showing that basal lamina-bound agrin from the central nervous system and that from non-neural tissues have similar molecular sizes. Agrin is stably but not covalently bound to the inner limiting membrane and could be completely removed only with strong detergents. Agrin could be partially extracted with buffers that are also able to partially release acetylcholine receptor aggregation activity from the neuromuscular junction or...Continue Reading

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Citations

Jul 11, 2000·The Journal of Comparative Neurology·H HeringS Kröger
Jan 9, 1998·Progress in Neurobiology·F Schmitz, D Drenckhahn
Oct 1, 1996·Current Opinion in Cell Biology·R Timpl
May 21, 1999·The European Journal of Neuroscience·M BlankS Kröger
Jul 17, 2008·Journal of Neurotrauma·M Cristina FaloLinda L Phillips
Nov 28, 2012·Cellular and Molecular Life Sciences : CMLS·Yinong Zong, Rongsheng Jin
Sep 26, 2015·Cellular and Molecular Life Sciences : CMLS·Mohammed M A AlmutairiHonglian Shi
Aug 11, 2006·Journal of Neurochemistry·Tatiana CheusovaSaid Hashemolhosseini
Jan 1, 1997·Molecular and Cellular Neurosciences·S Kröger
Sep 25, 2002·News in Physiological Sciences : an International Journal of Physiology Produced Jointly by the International Union of Physiological Sciences and the American Physiological Society·Stephan Kröger, Jörn E Schröder
Sep 9, 2017·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Zaynah MaherallyGeoffrey J Pilkington
Apr 22, 2004·Journal of Neuropathology and Experimental Neurology·Sònia SoléAnna M Planas
Mar 24, 2021·Experimental Eye Research·Kevin Y Zhang, Thomas V Johnson

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