Biochemical and genetic characterization of dengue virus methyltransferase

Virology
Hongping DongPei-Yong Shi

Abstract

We report that dengue virus (DENV) methyltransferase sequentially methylates the guanine N-7 and ribose 2'-O positions of viral RNA cap (GpppA-->m(7)GpppA-->m(7)GpppAm). The order of two methylations is determined by the preference of 2'-O methylation for substrate m(7)GpppA-RNA to GpppA-RNA, and the 2'-O methylation is not absolutely dependent on the prior N-7 methylation. A mutation that completely abolished the 2'-O methylation attenuated DENV replication in cell culture, whereas another mutation that abolished both methylations was lethal for viral replication, suggesting that N-7 methylation is more important than 2'-O methylation in viral replication. The latter mutant with lethal replication could be rescued by trans complementation using a wild-type DENV replicon. Furthermore, we found that chimeric DENVs containing the West Nile virus methyltransferase, polymerase, or full-length NS5 were nonreplicative, but the replication defect could also be rescued through trans complementation using the wild-type DENV replicon.

References

Jul 15, 1979·Virology·G R Cleaves, D T Dubin
Aug 1, 1982·Molecular and Cellular Biology·D WangA J Shatkin
May 22, 2002·Journal of Virology·Pei-Yong ShiKristen A Bernard
Aug 16, 2002·The Journal of Biological Chemistry·Jutta HagerUrsula Jakob
Jan 28, 2004·Nucleic Acids Research·Tricia M ColemanFaqing Huang
Mar 30, 2006·Antimicrobial Agents and Chemotherapy·Francesc Puig-BasagoitiPei-Yong Shi
Feb 3, 2007·Journal of Virology·Yangsheng ZhouHongmin Li
Feb 16, 2007·Journal of Virology·Hongping DongPei-Yong Shi
Jul 12, 2007·The Journal of General Virology·René AssenbergJonathan M Grimes
May 13, 2008·The Journal of Biological Chemistry·Helga KroschewskiAndrew D Davidson
Jun 24, 2008·Antiviral Research·Hongping DongPei-Yong Shi
Dec 23, 2008·Journal of Molecular Biology·Brian J GeissOlve B Peersen
Sep 4, 2009·Journal of Virology·Gang ZouPei-Yong Shi

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Citations

Dec 15, 2010·The Journal of Biological Chemistry·Siew Pheng LimPei-Yong Shi
Dec 25, 2010·The Journal of Biological Chemistry·Claudia V FilomatoriAndrea V Gamarnik
Apr 8, 2011·Journal of Virology·Nestor G IglesiasAndrea V Gamarnik
Mar 15, 2013·Journal of Virology·Shi-Hua LiPei-Yong Shi
Nov 19, 2015·Proceedings of the National Academy of Sciences of the United States of America·Yongqian ZhaoDahai Luo
Sep 23, 2014·Antiviral Research·Christian G NoblePei-Yong Shi
Mar 12, 2016·Expert Opinion on Drug Discovery·W Mei Kok
Feb 4, 2014·The Journal of General Virology·Hongping DongPei-Yong Shi
Apr 29, 2015·Antiviral Research·Siew Pheng LimPei-Yong Shi
Sep 22, 2012·Antiviral Research·Christian G Noble, Pei-Yong Shi
Oct 1, 2013·Antiviral Research·Siew Pheng LimPei-Yong Shi
Apr 13, 2012·PLoS Pathogens·Hongping DongPei-Yong Shi
Mar 27, 2015·PloS One·Hapuarachchige Chanditha HapuarachchiYee Sin Leo
Nov 4, 2015·Acta Crystallographica. Section D, Biological Crystallography·Wuan Geok SawGerhard Grüber
Sep 17, 2016·Cell Reports·Javier ColomaAneel K Aggarwal
Oct 18, 2016·European Journal of Medicinal Chemistry·Fatiha BenmansourKarine Barral
Apr 27, 2017·Viruses·Abbas El Sahili, Julien Lescar
May 17, 2012·Viruses·Juliet MorrisonAna Fernandez-Sesma
Dec 21, 2017·Frontiers in Microbiology·Shelton S Bradrick
Jan 15, 2011·Antiviral Chemistry & Chemotherapy·Justin G JulanderSujan Shresta
Dec 30, 2016·Journal of Virology·Bruno CoutardEtienne Decroly
Jun 7, 2013·Journal of Virology·Hyelim ChoMichael S Diamond
Mar 4, 2017·The EMBO Journal·Wenqian DuanGeorge F Gao
May 6, 2014·Journal of Biomolecular Screening·Shaun P Falk, Bernard Weisblum
Oct 25, 2017·Biotechnology Letters·Jae In JungSeong-Wook Lee
Oct 28, 2018·European Journal of Medicinal Chemistry·Jessica HernandezKarine Barral
Dec 29, 2020·RNA Biology·Alessia RuggieriLaurent Chatel-Chaix
May 24, 2020·Current Pharmaceutical Design·Venkatanarayana C MaddipatiRambabu Gundla

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