PMID: 19939227Nov 27, 2009Paper

Biochemical and histochemical comparison of cholinesterases in normal and Alzheimer brain tissues

Current Alzheimer Research
Sultan DarveshE Martin

Abstract

Cholinesterase activity associated with neuritic plaques (NPs) and neurofibrillary tangles (NFTs) in Alzheimer's disease (AD) brains exhibit altered histochemical properties, such as requiring lower pH (6.8) for optimal cholinesterase staining compared to the pH (8.0) for best visualization of cholinesterases in neurons. Furthermore, visualization of NPs and NFTs can be prevented by agents like the peptidase inhibitor/metalloantibiotic bacitracin. The anomalous behavior of cholinesterases associated with pathological lesions needs to be elucidated because of the putative links between these enzymes and the disease process in AD. In this study, cholinesterases were extracted from AD and normal brain tissue to determine whether the differences observed in histochemical analyses in the two sources were reflected in kinetic properties measured in solubilized enzymes. Isolated brain enzymes from both these sources exhibited comparable kinetic parameters with respect to pH dependence, substrate affinity and inhibitor sensitivity and were not significantly affected by other agents that blocked cholinesterase histochemical visualization, such as the structurally diverse metal-chelating antibiotics bacitracin, doxycycline, minocycline a...Continue Reading

Citations

Oct 27, 2010·Molecular Imaging and Biology : MIB : the Official Publication of the Academy of Molecular Imaging·Ian R MacdonaldSultan Darvesh
Dec 10, 2013·Journal of Molecular Neuroscience : MN·María-Letizia CampanariJavier Sáez-Valero
Jan 12, 2012·Journal of Enzyme Inhibition and Medicinal Chemistry·Ian R MacdonaldSultan Darvesh
Dec 14, 2011·Journal of Neuropathology and Experimental Neurology·Sultan DarveshChangiz Geula
Aug 30, 2011·Biochimica Et Biophysica Acta·Ryan WalshSultan Darvesh
Aug 27, 2015·Behavioural Brain Research·Tangui MauriceArnaud Chatonnet
May 28, 2013·Bioorganic & Medicinal Chemistry Letters·Sultan DarveshEarl Martin
Sep 1, 2012·Chemico-biological Interactions·Sultan Darvesh
Jun 24, 2017·Neuropathology and Applied Neurobiology·H HamodatS Darvesh
Apr 30, 2017·Journal of Alzheimer's Disease : JAD·Ian R MacdonaldSultan Darvesh
Oct 27, 2017·Alzheimer's & Dementia : Translational Research & Clinical Interventions·Drew R DeBaySultan Darvesh
Jun 10, 2019·Chemico-biological Interactions·Oksana GerlitsZoran Radić
Nov 7, 2015·Journal of Nuclear Medicine : Official Publication, Society of Nuclear Medicine·Ian R MacdonaldSultan Darvesh

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.