Biochemical and histochemical evidence of nonspecific binding of alpha7nAChR antibodies to mouse brain tissue

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
Donna L HerberMarcia N Gordon

Abstract

Alpha 7 nicotinic acetylcholine receptors are involved in learning and memory, and are implicated in the pathology of Alzheimer's disease and schizophrenia. Detection of alpha7 subunits can be accomplished via immunodetection or alpha-bungarotoxin-binding techniques. Standard protocols for immunohistochemistry and Western blotting were followed using several commercially available antibodies. Various mice were evaluated, including non-transgenics, APP, PS1, APP+PS1, and alpha7 knockouts. Initial results with amyloid-depositing mice revealed alpha7 immunolabeled astrocytes, in addition to expected neuronal staining. Subsequent studies with intrahippocampal injections of lipopolysaccharide (LPS) into alpha7 knockout mice showed that both neuronal and astrocytic labeling by alpha7 antibodies was nonspecific. On Western blots of mouse brain proteins, none of the bands detected with antibodies directed against alpha7 subunits diminished in the alpha7 knockout mice. Although LPS-related changes in the expression of some bands were found, these also were unaffected by the alpha7 genotype of the mice. In general, the Western staining patterns for these antibodies revealed few overlapping bands. These immunodetection data are in contras...Continue Reading

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Citations

May 25, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Nicolas WanaverbecqDimitri M Kullmann
Jun 21, 2008·Histochemistry and Cell Biology·W KummerU Pfeil
Jun 6, 2009·Acta Pharmacologica Sinica·Xuesi M Shao, Jack L Feldman
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Aug 15, 2014·BioMed Research International·Marion MeixnerVeronika Grau
Apr 9, 2008·Physiological Reviews·Balázs Lendvai, E Sylvester Vizi
Feb 13, 2015·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Frank R RommelEva M J Peters
Aug 7, 2013·Apoptosis : an International Journal on Programmed Cell Death·Octavian BucurRoya Khosravi-Far
Apr 13, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·S U SchirmerR V Haberberger
Oct 1, 2015·Pharmacological Reviews·Daniel BertrandDiana Donnelly-Roberts
Dec 13, 2012·Expert Opinion on Therapeutic Targets·Tanya L Wallace, Daniel Bertrand
Aug 2, 2017·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Brijesh K Garg, Ralph H Loring
Jul 25, 2021·Pharmacological Reviews·Roger L Papke, Nicole A Horenstein
Oct 5, 2018·Journal of Proteome Research·Matthew J MulcahyEdward Hawrot
Aug 18, 2009·Neuroscience Research·John G Mielke, Geoffrey A R Mealing

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