Abstract
Recently we demonstrated that the keratin 17 (K17) content exceeded the K16 content in most follicular tumors, in comparison with non-follicular epithelial skin tumours by two-dimensional gel electrophoresis (2-DE), densitometry and immunohistochemistry. At present the origin of basal cell carcinoma (BCC) is unknown. So, based on the above results, we studied keratin expression in eight cases of BCC, in order to analyze tumor differentiation by both biochemical and immunohistochemical methods. Biochemically, using 2-DE and immunoblotting, stratified epithelial keratins K5/K14 and large amounts of K17 were present in all cases. Simple epithelial keratins K8 and K19 were expressed in all and half of the cases, respectively. However, hyperproliferative associated keratins (K6/K16) and keratinized keratins (K1/K10) were detected in only a few cases. Immunohistochemical studies using frozen sections with chain-specific antikeratin monoclonal antibodies against K1, K7, K8, K10, K14, K16, K17, K18 and K19 showed that BCC tumor cells reacted positively with antibodies against K8, K14, K17 and K19, but did not react, or were rarely positive with K1, K7, K10, K16 and K18 antibodies. Predominant expression of K17 and the frequent expressi...Continue Reading
References
Dec 1, 1977·Cell·P Z O'FarrellP H O'Farrell
Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J O Thomas, R D Kornberg
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Feb 1, 1992·The British Journal of Dermatology·A C MarkeyI M Leigh
May 1, 1990·Carcinogenesis·W BurschR Schulte-Hermann
Jul 1, 1986·The Journal of Cell Biology·A SchermerT T Sun
Jan 1, 1986·Differentiation; Research in Biological Diversity·M HuszarB Geiger
Nov 1, 1986·The Journal of Cell Biology·A L Tyner, E Fuchs
Jan 1, 1987·Differentiation; Research in Biological Diversity·H J StarkN E Fusenig
Jan 1, 1988·Differentiation; Research in Biological Diversity·H W HeidW W Franke
Aug 1, 1988·The Journal of Cell Biology·A StolerE Fuchs
Jan 1, 1989·Archives of Dermatological Research·A P LavrijsenG N van Muijen
Dec 1, 1989·The American Journal of Dermatopathology·N ShimizuY Sato
May 1, 1972·The Journal of Pathology·J F Kerr, J Searle
Aug 15, 1970·Nature·U K Laemmli
Oct 1, 1982·The Journal of Cell Biology·R MollR Krepler
Nov 1, 1982·Cell·R MollR Krepler
Jan 1, 1982·Differentiation; Research in Biological Diversity·R MollW Wiest
Jan 1, 1993·Archives of Dermatological Research·K YoshikawaS Kondo
May 1, 1996·Archives of Dermatological Research·O MoriY Sasai
Jun 1, 1953·A.M.A. Archives of Dermatology and Syphilology·H PINKUS
Apr 1, 1948·Archives of Dermatology and Syphilology·W F LEVER
Citations
Apr 2, 2011·Analytical and Bioanalytical Chemistry·Riccardo Cicchi, Francesco Saverio Pavone
Sep 2, 2004·Archives of Dermatological Research·Gang WangYan Wang
Jan 26, 2010·Transgenic Research·Amy C McCalla-MartinJorge A Piedrahita
Jun 28, 2001·Journal of Cutaneous Pathology·X ChenT Kanzaki
Feb 13, 2001·Journal of Cutaneous Pathology·L M LaoK Ueda
Aug 14, 2002·The British Journal of Dermatology·H TakedaS Kondo
May 16, 2002·Histopathology·P G Chu, L M Weiss
Jul 8, 2000·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·S GibbsM Ponec
Mar 20, 2009·Molecular & Cellular Proteomics : MCP·Lang-Ming ChiJau-Song Yu
Jun 12, 2002·Molecular Biology of the Cell·Diana M ToivolaM Bishr Omary
Jun 14, 2012·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Heidi Anderson-DockterVincent Falanga
Oct 20, 2007·Oral Diseases·M KishinoS Toyosawa
Sep 2, 2011·Molecular Carcinogenesis·Karin Heby-HenricsonStephan Teglund
Jun 30, 2011·The British Journal of Dermatology·B K K LoK J McElwee
Dec 7, 2010·The British Journal of Dermatology·K Sellheyer
Apr 9, 2016·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Francesca AbramoVincenzo Miragliotta
Mar 17, 2010·The American Journal of Pathology·Blanche Ka Ki LoKevin John McElwee
Mar 5, 2015·Virchows Archiv : an International Journal of Pathology·Toshihiko MikamiTakashi Saku
Feb 26, 2016·The British Journal of Dermatology·S R QuistH P Gollnick
May 16, 2012·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Maja EydnerWolfgang Baumgärtner
Mar 4, 2000·The American Journal of Dermatopathology·P J Lespi, S D Gregorini
Nov 28, 2012·Nature Cell Biology·Khalil Kass YoussefCédric Blanpain
Dec 16, 2011·Experimental Dermatology·Martin WagnerKamil Onder
Feb 12, 2004·Dermatologic Surgery : Official Publication for American Society for Dermatologic Surgery [et Al.]·Roddie C McKenzie, Jacek Szepietowski
Feb 19, 2019·Biomedical Optics Express·Xu FengJames W Tunnell
Feb 17, 2021·Molecular Medicine·Anne MenzRonald Simon