PMID: 9525776Apr 3, 1998Paper

Biochemical and immunological analyses of the flagellin of Clostridium tyrobutyricum ATCC 25755

Microbiology and Immunology
F ArnoldR Malcoste

Abstract

The monoclonal antibody 21E7-B12 (IgG3) can be used in a direct method of Clostridium tyrobutyricum detection based on an immunoenzymatic assay. Immunoelectron microscopy demonstrated that the 21E7-B12 antibody recognized the surface-exposed epitopes on the flagellar filaments of C. tyrobutyricum. After flagellar extraction, the purified flagellin showed an apparent molecular mass of 46 kDa with an isoelectric point of 3.6. Sugar staining, mild periodate oxidation and beta-elimination experiments showed that the flagellin was glycosylated and that the 21E7-B12 epitope was located in the sugar moiety. Amino acid composition showed that the flagellar filament protein contained a high percentage of serine and threonine, while proline was absent. The first 23 residues of the N-terminal were determined and sequence homology with other flagellins was found.

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Citations

Aug 23, 2007·Inflammatory Bowel Diseases·L Wayne DuckCharles O Elson
Jul 27, 1999·Veterinary Microbiology·A KojimaY Tamura
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·S K AroraR Ramphal
Mar 14, 2007·Applied and Environmental Microbiology·Catherine J PaulSusan M Logan
May 2, 2000·Applied and Environmental Microbiology·N CharniN Rugani
Sep 15, 2009·Journal of Bacteriology·Susan M TwineSusan M Logan
Aug 2, 2008·The FEBS Journal·Susan M TwineSusan M Logan
Aug 8, 2007·Veterinary Microbiology·Tulay I Cagatay, Jon G H Hickford
Sep 4, 2014·Microbiology and Molecular Biology Reviews : MMBR·Hanne L P Tytgat, Sarah Lebeer
Apr 28, 2000·Microbiology·A TasteyreP Borriello
Sep 26, 2015·Glycobiology·Bettina JaneschChristina Schäffer
Oct 21, 2016·The Journal of Biological Chemistry·Laura BouchéHoward R Morris

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