Abstract
The gene coding for a dioxygenase with the ability to cleave salicylate by a direct ring fission mechanism to 2-oxohepta-3,5-dienedioic acid was cloned from Pseudaminobacter salicylatoxidans strain BN12. The deduced amino acid sequence encoded a protein with a molecular mass of 41,176 Da, which showed 28 and 31% sequence identity, respectively, to a gentisate 1,2-dioxygenase from Pseudomonas alcaligenes NCIMB 9867 and a 1-hydroxy-2-naphthoate 1,2-dioxygenase from Nocardioides sp. KP7. The highest degree of sequence identity (58%) was found to a presumed gentisate 1,2-dioxygenase from Corynebacterium glutamicum. The enzyme from P. salicylatoxidans BN12 was heterologously expressed in Escherichia coli and purified as a His-tagged enzyme variant. The purified enzyme oxidized in addition to salicylate, gentisate, 5-aminosalicylate, and 1-hydroxy-2-naphthoate also 3-amino- and 3- and 4-hydroxysalicylate, 5-fluorosalicylate, 3-, 4-, and 5-chlorosalicylate, 3-, 4-, and 5-bromosalicylate, 3-, 4-, and 5-methylsalicylate, and 3,5-dichlorosalicylate. The reactions were analyzed by high pressure liquid chromatography/mass spectrometry, and the reaction products were tentatively identified. For comparison, the putative gentisate 1,2-dioxyge...Continue Reading
References
Mar 1, 1975·Journal of Bacteriology·R L CrawfordP J Chapman
Sep 1, 1979·Applied and Environmental Microbiology·R L CrawfordT D Frick
May 7, 1976·Analytical Biochemistry·M M Bradford
Mar 15, 1992·The Biochemical Journal·A StolzH J Knackmuss
Mar 11, 1991·Nucleic Acids Research·D MarchukF S Collins
Jul 1, 1986·Archives of Microbiology·M A RubioH J Knackmuss
Jan 1, 1974·Archives of Microbiology·E DornH J Knackmuss
Aug 15, 1970·Nature·U K Laemmli
Sep 15, 1996·FEMS Microbiology Letters·M SuárezM Martín
Sep 1, 1996·Molecular Microbiology·J N VolffJ Altenbuchner
Nov 1, 1996·Journal of Bacteriology·U Lendenmann, J C Spain
Jun 6, 1997·The Journal of Biological Chemistry·S TakenakaK Aoki
Jun 1, 1997·BioTechniques·K M Pang, D A Knecht
Feb 14, 1998·European Journal of Biochemistry·E L Hegg, L Que
May 9, 1998·The Journal of Biological Chemistry·T Iwabuchi, S Harayama
Apr 16, 1998·FEBS Letters·R KucharczykC J Herbert
Jul 31, 1998·Archives of Microbiology·S TakenakaK Aoki
Aug 8, 1998·Journal of Bacteriology·J WerwathR M Wittich
Nov 25, 1998·Extremophiles : Life Under Extreme Conditions·W Fu, P Oriel
Jan 28, 1999·Journal of Bacteriology·K AdachiS Harayama
Mar 2, 1999·Applied and Environmental Microbiology·Y FengC L Poh
May 13, 1999·International Journal of Systematic Bacteriology·P KämpferA Stolz
Nov 7, 1999·Archives of Microbiology·J K DavisJ C Spain
Jul 6, 2000·Nature Structural Biology·G P TitusD E Timm
Jan 3, 2001·Journal of Bacteriology·N Y ZhouP A Williams
Jul 14, 2001·Critical Reviews in Microbiology·V Mishra Srinivasan
Oct 30, 2001·FEMS Microbiology Letters·C H ChuaC L Poh
Nov 8, 2001·Journal of Bacteriology·J P HintnerA Stolz
Nov 7, 1996·Chemical Reviews·Lawrence Que, Raymond Y. N. Ho
Citations
Sep 29, 2011·Biochemistry·Timothy E Machonkin, Amy E Doerner
Nov 1, 2011·Inorganic Chemistry·Heaweon ParkAdam T Fiedler
Aug 30, 2012·Dalton Transactions : an International Journal of Inorganic Chemistry·Amanda E BaumAdam T Fiedler
Aug 24, 2012·The Journal of Biological Chemistry·Liangtao LiJerry Kaplan
Jun 7, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·I MateraF Briganti
Jan 19, 2010·Applied and Environmental Microbiology·Yi Qu, Jim C Spain
Jan 31, 2012·Applied and Environmental Microbiology·Susanne Fetzner
Jul 8, 2005·Applied and Environmental Microbiology·Xi-Hui ShenShuang-Jiang Liu
Apr 19, 2011·Journal of Bacteriology·Yi Qu, Jim C Spain
May 3, 2014·Inorganic Chemistry·Biswarup ChakrabortyTapan Kanti Paine
Jan 11, 2007·Bioscience, Biotechnology, and Biochemistry·Shin-ichi HiranoShigenori Kanaya
Jun 14, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Stefano Da VelaFabrizio Briganti
Nov 20, 2009·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Timothy E MachonkinSara S Rocks
Sep 18, 2010·Critical Reviews in Microbiology·Somnath MallickTapan K Dutta
Jun 24, 2008·Journal of Molecular Biology·Irene MateraFabrizio Briganti
Nov 6, 2015·FEMS Microbiology Letters·Erik EppingerAndreas Stolz
Aug 26, 2006·Molecular Microbiology·Melanie A AdamsZongchao Jia
Feb 7, 2013·The FEBS Journal·Marta FerraroniFabrizio Briganti
Jul 4, 2009·Microbiology·Jaigeeth Deveryshetty, Prashant S Phale
Jul 29, 2015·Applied Microbiology and Biotechnology·Martina Kiel, Karl-Heinrich Engesser
Jun 21, 2015·Biochimica Et Biophysica Acta·Erik EppingerAndreas Stolz
Dec 14, 2011·Journal of Structural Biology·Marta FerraroniFabrizio Briganti
Sep 11, 2012·Journal of Structural Biology·Marta FerraroniFabrizio Briganti
Oct 19, 2017·Journal of Bacteriology·Hao YuLizhong Guo
Nov 26, 2016·Protein Engineering, Design & Selection : PEDS·Erik Eppinger, Andreas Stolz
Aug 4, 2019·Environmental Science and Pollution Research International·Estefania BraccoRoberto Candal
Feb 4, 2019·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Aleksey AleshintsevRupal Gupta
Jun 8, 2013·Chemistry : a European Journal·Michael M BittnerAdam T Fiedler
Oct 31, 2020·Biochimie·Natalya M SubbotinaMarina P Kolomytseva