PMID: 9165096Apr 25, 1997Paper

Biochemical and molecular characterization of a manganese peroxidase isoenzyme from Pleurotus ostreatus

Biochimica Et Biophysica Acta
S SarkarM J Martínez

Abstract

In this study we purified and investigated the catalytic properties of a manganese peroxidase isoenzyme produced by the fungus Pleurotus ostreatus in liquid medium with peptone as nitrogen source. The isoenzyme was purified to homogeneity by chromatography on Bio-Rad Q-cartridge, Sephacryl S-200 and Mono-Q with activity yield of 59% and a purification factor of 36. The P. ostreatus MnP obtained had the same pI (3.75) and N-terminal sequence as MnP-1 of Pleurotus eryngii produced in the same medium (both exhibiting Mn-independent activities on phenolic and non-phenolic substrates). However, the N-terminal sequence of this P. ostreatus isoenzyme differed from a previous published sequence of MnP from this fungus. The results obtained show the importance of media composition in the production of different isoenzymes within the same fungal species. We have also demonstrated by Southern blots that the different isoenzymes are probably encoded by different genes, and that the MnP genes in both Pleurotus species are similar but different to those of Phanerochaete chrysosporium.

Citations

Jul 8, 2010·Applied Microbiology and Biotechnology·A GoudopoulouMilton A Typas
Nov 19, 2003·Biotechnology Advances·Dirk WesenbergSpiros N Agathos
Mar 11, 2008·Applied and Environmental Microbiology·Takahisa TsukiharaTakashi Watanabe
Dec 4, 2012·Enzyme and Microbial Technology·Natalia PozdnyakovaAnna Jarosz-Wilkolazka
Dec 4, 2012·Enzyme and Microbial Technology·Grzegorz JanuszAndrzej J Paszczynski
Jan 15, 2005·Biochemical and Biophysical Research Communications·Hisatoshi KamitsujiMasaaki Kuwahara
Jan 9, 2015·Environmental Science and Pollution Research International·Maria Camilla BarattoRafael Vazquez-Duhalt
Sep 6, 2003·Biological Chemistry·Holger ZornRalf G Berger
Apr 17, 2013·Biotechnology and Applied Biochemistry·Hirofumi Ichinose
Apr 11, 2019·PeerJ·Alona S SekanYaroslav Blume
Mar 2, 1999·Applied and Environmental Microbiology·B BockleA T Martinez
Jul 2, 1998·Applied and Environmental Microbiology·M T MoreiraJ A Field
Oct 3, 1999·Applied and Environmental Microbiology·F J Ruiz-DueñasA T Martínez
Jul 31, 1999·Applied and Environmental Microbiology·L M LarrayaL Ramírez
Mar 24, 2000·Archives of Biochemistry and Biophysics·P GiardinaG Sannia
Aug 16, 2021·Journal of Colloid and Interface Science·Mohamed S SelimPing Jing Mo

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