Biochemical and molecular characterization of Cronobacter spp. (formerly Enterobacter sakazakii) isolated from foods

Antonie van Leeuwenhoek
Imrich TurcovskýEva Kaclíková

Abstract

The aim of this study was to identify and characterize Cronobacter spp. isolated from a range of foods. A total of 71 Cronobacter strains were isolated from 602 foods in our laboratory. The highest contamination was observed in foods of plant origin, e.g. spices, teas, chocolate, nuts, pastries and vegetables. On the basis of genus and species identification performed using genus-specific PCR, 16S rRNA sequencing and AFLP genotyping, most of the strains belonged to Cronobacter sakazakii. Biochemical profiling by the tests included in API 20E, complemented with relevant additional tests, classified the strains into 13 biogroups. AFLP genotyping facilitated discrimination of six main groups at the 70% similarity level and strain grouping correlated clearly with species identification. Our results indicate that molecular typing by AFLP may be applied as a useful tool not only for direct comparison of Cronobacter isolates, providing traceability, but also for the reliable species classification. Moreover, tracing of these bacteria in a wider variety of foods should be important to enhance the knowledge of their transmission.

References

Jan 1, 1997·Letters in Applied Microbiology·M Nazarowec-White, J M Farber
Nov 25, 2003·Journal of Applied Microbiology·P BreeuwerH M Joosten
Jan 16, 2004·Lancet·Jeffrey M Farber
Jan 16, 2004·Lancet·M Chantal KandhaiMike van Schothorst
Sep 15, 2004·International Journal of Food Microbiology·Carol IversenStephen Forsythe
Jul 26, 2005·International Journal of Food Microbiology·Joshua B GurtlerLarry R Beuchat
Sep 16, 2005·Journal of Food Protection·Sharon G Edelson-MammelRobert L Buchanan
May 30, 2006·International Journal of Food Microbiology·Denise DrudySéamus Fanning
Oct 28, 2006·BMC Microbiology·Carol IversenStephen J Forsythe
Oct 31, 2006·Applied and Environmental Microbiology·Carol Iversen, Stephen J Forsythe
Mar 3, 2007·International Journal of Food Microbiology·Miriam Friedemann
May 10, 2007·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Sep 7, 2007·ANZ Journal of Surgery·Pallab RayMeera Sharma
Mar 4, 2008·Applied and Environmental Microbiology·Carol IversenRoger Stephan
Feb 4, 2009·BMC Microbiology·Walid M El-SharoudBrendan Healy
Mar 27, 2009·International Journal of Food Microbiology·Peter KuhnertCarol Iversen
Sep 2, 2009·International Journal of Food Microbiology·T Osaili, S Forsythe
Sep 5, 2009·International Journal of Food Microbiology·J ChapS J Forsythe
Sep 15, 2009·Research in Microbiology·Michael SchmidAngelika Lehner
Nov 10, 2009·International Journal of Food Microbiology·Patrick Druggan, Carol Iversen

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Citations

Sep 20, 2012·Foodborne Pathogens and Disease·Cristina ArroyoSantiago Condón
Jul 13, 2012·Journal of Clinical Microbiology·S JosephS J Forsythe
Jun 29, 2011·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Lubomíra TóthováJúlius Hodosy
Mar 12, 2013·Molecular and Cellular Probes·Susan JosephStephen J Forsythe
Oct 22, 2013·Letters in Applied Microbiology·A GičováE Kaclíková
Jan 28, 2014·Journal of Microbiological Methods·Lukáš KrásnýIgor Hochel
Apr 8, 2015·Food Microbiology·M GarbowskaL Stasiak-Różańska
Dec 18, 2013·The Journal of Hospital Infection·O Holý, S Forsythe
Nov 5, 2016·International Journal of Biological Macromolecules·Yinglian Zhu, Dongfeng Wang
Dec 23, 2017·Annual Review of Food Science and Technology·Stephen J Forsythe
Dec 23, 2017·Antonie van Leeuwenhoek·Veronika KadlicekovaHana Drahovska
Aug 12, 2014·Science Progress·Ben D TallKeith A Lampel
Sep 19, 2018·Emerging Infectious Diseases·Maria Del Rocío Morato-RodríguezHerbert Vera
Mar 27, 2020·Frontiers in Microbiology·Amal A ElkhawagaMohamed A El-Mokhtar

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