Biochemical and molecular identification and characterization of lactic acid bacteria and yeasts isolated from Ethiopian naturally fermented buttermilk

Journal of Food Science and Technology
Negussie GebreselassieFekadu Beyene

Abstract

Lactic acid bacteria (LAB) and yeasts were enumerated and identified from naturally fermented buttermilk. Isolates were first subjected to chemical tests and then to molecular characterization. Molecular identification involved pure sequencing of 16s rRNA (LAB) and 18s rRNA (yeast) genes. Polymerase Chain Reaction-Denaturing Gradient Gel Electrophoresis (DGGE) was used for detection of microbiota composition. Eighty-five LAB and 26 yeast isolates obtained from 16 small-scale dairy farms were isolated and identified. The microbial composition was dominated by strains of Lactococcus lactis ssp. lactis, Lactobacillus plantarum and Saccharomyces cerevisiae. Molecular techniques enabled not only genetic confirmation but also detection of some uncultivated strains. The presence of diverse strains of LAB and yeasts in NFB indicated a potential for development of different starter cultures to make new dairy products.

References

Nov 1, 1990·Applied and Environmental Microbiology·R J RouwenhorstJ P van Duken
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1996·International Journal of Food Microbiology·R Roostita, G H Fleet
Jun 29, 1999·Current Opinion in Microbiology·G Muyzer
Jul 29, 2000·FEMS Microbiology Letters·L CocolinD A Mills
Mar 15, 2001·International Journal of Food Microbiology·E M BeukesJ F Mostert
Sep 1, 2001·International Journal of Food Microbiology·A GonfaW H Holzapfel
Apr 3, 2002·Current Microbiology·Danyelly Bruneska Gondim MartinsMarcos Antonio de Morais
May 31, 2002·International Journal of Food Microbiology·W H Holzapfel
Sep 5, 2009·Current Microbiology·Akihito EndoL M T Dicks
Oct 18, 2013·Molecular Biology and Evolution·Koichiro TamuraSudhir Kumar
Jun 1, 1990·World Journal of Microbiology & Biotechnology·S B Feresu, M I Muzondo
Jan 22, 2014·Food Chemistry·Smita S Zinjarde

❮ Previous
Next ❯

Citations

Sep 11, 2018·Frontiers in Microbiology·Shannon RezacRobert Hutkins
Nov 20, 2020·Critical Reviews in Food Science and Nutrition·Gilberto V de Melo PereiraCarlos R Soccol
Aug 8, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anshula SharmaBaljinder Kaur

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.