Abstract
Tea (Camellia sinensis) plantations are exposed to biotic and abiotic stresses. Among the biotic factors, blister blight (BB), caused by Exobasidium vexans, affects the quality and quantity of the product and demands high fungicide application. A long term solution for disease resistance would require the knowledge of the basic molecular and biochemical changes occurring in plant as an attempt to resist the pathogen and limit the spread of the disease which can further help in developing resistant cultivars using biotechnological tools. Thus, gene expression studies using the cDNA based suppressive subtractive hybridization library, characterization of genes for pathogenesis related (PR) proteins [chitinase (CsCHIT), glucanase (CsGLUC), phenylalanine ammonia lyase (CsPAL)] and genes in flavonoid pathway were accessed in the BB resistant and susceptible cultivars, SA6 and TES34, respectively. Further, biochemical analysis of PR and antioxidant enzymes (POX, APX, SOD) involved in BB resistance have been carried out to investigate the potential molecular and biochemical changes. Various stages of pathogen development had varied impact on PR protein, flavonoid pathway and anti-oxidative enzymes and indicates the possible role of re...Continue Reading
References
Jan 1, 1996·International Orthopaedics·T J ChesserA J Ward
Nov 26, 1999·Proceedings of the National Academy of Sciences of the United States of America·R MittlerB E Ellis
Jun 19, 2001·Molecular Genetics and Genomics : MGG·J ThimmapuramS S Korban
Oct 11, 2001·Biological Chemistry·K Zwerger, H Hirt
Jun 14, 2003·Plant Physiology·Nicholas J ProvartTong Zhu
Oct 27, 2004·Phytochemistry·P A Nimal PunyasiriN H L Pradeepa
Dec 3, 2005·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Laszlo Tretter, Vera Adam-Vizi
Jul 6, 2006·Biotechnology Letters·Yuying SunBing Liu
Jul 3, 2007·Molecular Plant-microbe Interactions : MPMI·Manoela MirandaC Peter Constabel
Nov 21, 2007·Antioxidants & Redox Signaling·Weihai Ying
Dec 5, 2008·Proteomics·Ivy WidjajaJustin Lee
May 1, 2007·Molecular Plant Pathology·Dawn Foster-HartnettDeborah A Samac
Apr 13, 2011·Plant Physiology and Biochemistry : PPB·Thirugnanasambantham KrishnarajAbul Kalam Azad Mandal
Aug 2, 2012·Biotechnology Letters·Vaiyapuri BalasubramanianNatarajan Sakthivel
Oct 16, 2012·Applied Biochemistry and Biotechnology·Palanisamy SenthilkumarAbul Kalam Azad Mandal
Sep 27, 2013·Applied Biochemistry and Biotechnology·Senthilkumar Palanisamy, Abul Kalam Azad Mandal
Jan 1, 2013·Metabolites·Jordan D RadomiljacMargaretha van der Merwe
Mar 17, 2015·Functional & Integrative Genomics·H Ranjit SinghSudripta Das