Biochemical and morphological characteristics of selenite-induced apoptosis in human hepatoma Hep G2 cells

Biological Trace Element Research
Handan Ak CelikYucel Batur

Abstract

Selenium is a cellular growth inhibitor in many mammary tumor cells. To comprehend the mechanism for the selenium-induced cell death, we examined the effects of sodium selenite, which has been one of the most extensively investigated selenium compounds, in human hepatoma Hep G2 cells.Cell viability gradually decreased after treatment with sodium selenite within the concentration range of 10-50 microM. Low (10 mM) selenite has shown a high-percentage laddering pattern compared to the high (25 microM) cytotoxic selenium concentration in agarose gel electrophoresis. G2/M-phase enrichment was also concentration dependent. The most consistent transmission electron microscopic finding was the existence of large lysosomes. Based on these data, we hypothesize that sodium selenite predominantly shows its apoptotic effect over hydrogen selenite accumulation.

Citations

Sep 18, 2007·Biological Trace Element Research·Handan Ak CelikHimet Hakan Aydin
Jul 16, 2014·Journal of Trace Elements in Medicine and Biology : Organ of the Society for Minerals and Trace Elements (GMS)·Shauna M McKelveyRichard A Murphy
Aug 5, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Sougat Misra, Som Niyogi
Dec 3, 2014·Biochimica Et Biophysica Acta·Aristi P Fernandes, Valentina Gandin
May 1, 2009·Environmental Toxicology and Pharmacology·Rolf Gebhardt
Nov 15, 2005·Diabetes/metabolism Research and Reviews·Murat AyazBelma Turan

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis