Biochemical and physiologic characterization of follicle regulatory protein: a paracrine regulator of folliculogenesis

American Journal of Obstetrics and Gynecology
T OnoG S diZerega

Abstract

Further purification of a porcine follicular fluid fraction, referred to as follicle regulatory protein, that inhibits granulosa cell aromatase was performed and the results of in vitro bioassays with these highly purified reagents are reported. The 0% to 35% saturated ammonium sulfate extract of porcine follicular fluid was percolated through an orange A dye matrex gel column and the bound fraction was eluted. Further purification of 0% to 35% orange A-bound fraction of porcine follicular fluid was performed by anion exchange chromatography with the use of the Mono Q column. Mono Q eluents containing follicle regulatory protein activity were injected onto a Mono P hydrogen ion-exchange column. Samples obtained from Mono P chromatography were injected onto preparative and analytical scale gel exclusion columns. Eluent fractions in the apparent molecular weight of 16,000 daltons were tested for aromatase inhibition. Throughout each step, parallelism of an aromatase inhibitor was apparent in both a cell-free microsomal assay and a granulosa cell assay. Follicle regulatory protein, purified about 6666-fold from the orange A-bound fraction of porcine follicular fluid, had a 50% inhibitory concentration of 25 ng/ml for granulosa cel...Continue Reading

References

Dec 1, 1986·American Journal of Obstetrics and Gynecology·J R Schreiber, G S diZerega
Aug 1, 1986·Experimental and Clinical Endocrinology·J D CampeauG S diZerega
Jan 1, 1983·The Journal of Clinical Endocrinology and Metabolism·G S diZeregaO R Kling
Jan 1, 1983·The Journal of Clinical Endocrinology and Metabolism·G S diZeregaO R Kling
Jun 1, 1982·The Journal of Clinical Endocrinology and Metabolism·G S diZeregaR M Nakamura

❮ Previous
Next ❯

Citations

Jan 1, 1987·Journal of Steroid Biochemistry·G S DiZeregaG Westhof
Jul 2, 2003·Journal of Biochemical and Biophysical Methods·Patrick VincentXavier Santarelli
Dec 1, 1986·American Journal of Obstetrics and Gynecology·J R Schreiber, G S diZerega

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.