Biochemical and quantitative analysis of Tamm Horsfall protein in rats

Urological Research
J A GokhaleS R Khan

Abstract

The involvement of Tamm Horsfall protein (THP) in nephrolithiasis is currently under investigation in several laboratories. Although rat is a commonly used species as an in vivo model for such studies, there is only limited information available about the biochemical properties and excretion profile of THP in normal rats. In order to characterize rat THP, we purified and analyzed normal male rat THP, and compared it with normal human male urinary THP by gel electrophoresis. Both THPs migrated at approximately 90 KDa, and stained similarly for protein (Coomassie blue) as well as carbohydrates (periodic acid Schiff reagent). Compositional analysis revealed that rat THP was largely similar to human THP in amino acid and carbohydrate contents but showed differences in the individual sugar components from other mammals. There was considerable variation in the day-to-day urinary excretion of THP in normal rats, with values ranging from 552.96 micrograms to 2865.60 micrograms and a mean value of 1679.54 micrograms per 24 h. It was concluded from this study that rat THP did not contain any unusual biochemical components and was primarily similar to human THP in composition and mean urinary concentration.

References

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Citations

Jul 27, 2010·Urological Research·Saeed R Khan
Jan 14, 2011·Urological Research·Pragasam ViswanathanJeffrey A Wesson
Apr 28, 2005·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·Kamalanathan SumitraPalaninathan Varalakshmi
Jan 8, 2005·Nephron. Physiology·Viswanathan PragasamPalaninathan Varalakshmi
Oct 24, 2013·BioMed Research International·Kanu Priya AggarwalChanderdeep Tandon
Jun 13, 2006·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Viswanathan PragasamPalaninathan Varalakshmi
Jun 7, 2000·The Journal of Urology·S MaslamaniS R Khan
Sep 8, 2001·The Journal of Urology·J A GokhaleS R Khan
Jul 5, 2005·Clinica Chimica Acta; International Journal of Clinical Chemistry·Viswanathan PragasamPalaninathan Varalakshmi
Jun 11, 2010·American Journal of Physiology. Renal Physiology·Yan LiKenneth E McMartin

Related Concepts

UMOD protein, human
Umod protein, rat
Metazoa
Sugars
SDS-PAGE
Antibody Dissociation
Mucoproteins
Silver
Staining and Labeling
Western Blot

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