Biochemical and spectroscopic characterization of the human mitochondrial amidoxime reducing components hmARC-1 and hmARC-2 suggests the existence of a new molybdenum enzyme family in eukaryotes

The Journal of Biological Chemistry
Bettina WahlFlorian Bittner


The mitochondrial amidoxime reducing component mARC is a newly discovered molybdenum enzyme that is presumed to form the catalytical part of a three-component enzyme system, consisting of mARC, heme/cytochrome b(5), and NADH/FAD-dependent cytochrome b(5) reductase. mARC proteins share a significant degree of homology to the molybdenum cofactor-binding domain of eukaryotic molybdenum cofactor sulfurase proteins, the latter catalyzing the post-translational activation of aldehyde oxidase and xanthine oxidoreductase. The human genome harbors two mARC genes, referred to as hmARC-1/MOSC-1 and hmARC-2/MOSC-2, which are organized in a tandem arrangement on chromosome 1. Recombinant expression of hmARC-1 and hmARC-2 proteins in Escherichia coli reveals that both proteins are monomeric in their active forms, which is in contrast to all other eukaryotic molybdenum enzymes that act as homo- or heterodimers. Both hmARC-1 and hmARC-2 catalyze the N-reduction of a variety of N-hydroxylated substrates such as N-hydroxy-cytosine, albeit with different specificities. Reconstitution of active molybdenum cofactor onto recombinant hmARC-1 and hmARC-2 proteins in the absence of sulfur indicates that mARC proteins do not belong to the xanthine oxida...Continue Reading


Jan 1, 1978·Methods in Enzymology·K Mihara, R Sato
Jan 1, 1978·Methods in Enzymology·R W Estabrook, J Werringloer
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J L Johnson, K V Rajagopalan
Aug 1, 1982·Journal of Bacteriology·V Stewart, C H MacGregor
Aug 24, 1995·Biochemical and Biophysical Research Communications·T E BatesJ B Clark
Jan 1, 1997·Annual Review of Biochemistry·C KiskerD C Rees
Oct 27, 1997·The Journal of Biological Chemistry·Günter SchwarzRalf R Mendel
Apr 17, 2001·Biochemical and Biophysical Research Communications·K IchidaT Nishino
Nov 7, 1996·Chemical Reviews·Russ Hille
Jul 17, 2003·The Journal of Biological Chemistry·Sandrine Da CruzJean-Claude Martinou
Jun 1, 1953·The Biochemical Journal·L G WHITBY
Oct 25, 2003·Proceedings of the National Academy of Sciences of the United States of America·Francisco Rodríguez-TrellesFrancisco J Ayala
Dec 8, 2004·Archives of Biochemistry and Biophysics·Russ Hille
Sep 29, 2005·Journal of Magnetic Resonance·Stefan Stoll, Arthur Schweiger
Jun 21, 2006·Biochimica Et Biophysica Acta·Ralf R Mendel, Florian Bittner
Jul 29, 2006·The Journal of Biological Chemistry·Katrin FischerGuenter Schwarz
Mar 4, 2008·Molecular Microbiology·Stanislav G KozminRoel M Schaaper
May 20, 2008·Journal of Molecular Biology·Yan Zhang, Vadim N Gladyshev
Aug 14, 2009·Nature·Günter SchwarzMarkus W Ribbe
Aug 12, 2010·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Antje HavemeyerBernd Clement


Jun 11, 2011·Plant Cell Reports·Ralf R Mendel
May 21, 2013·Chemical Research in Toxicology·Kai Wang, F Peter Guengerich
May 31, 2011·Metallomics : Integrated Biometal Science·Angel LlamasAurora Galvan
Jun 27, 2013·Metallomics : Integrated Biometal Science·Manuel Tejada-JimenezAngel Llamas
Jan 29, 2014·Chemical Reviews·Russ HillePartha Basu
Sep 29, 2011·Drug Metabolism Reviews·Antje HavemeyerBernd Clement
Dec 15, 2015·Molecular Genetics and Metabolism·Paldeep S Atwal, Fernando Scaglia
Oct 3, 2013·Molecular Systems Biology·Yuval TabachGary Ruvkun
Jul 31, 2013·MicrobiologyOpen·Stanislav G KozminRoel M Schaaper
Jun 20, 2014·Free Radical Biology & Medicine·Anna B NikiforovaAlexey G Kruglov
Feb 6, 2013·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Danilo FroriepAntje Havemeyer
Nov 27, 2014·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Gudrun OttBernd Clement
Jan 16, 2015·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Luisa B Maia, José J G Moura
Sep 18, 2015·PloS One·Etienne P A NeveMagnus Ingelman-Sundberg
Oct 1, 2016·Redox Report : Communications in Free Radical Research·Jun WangZhiyuan Mi
Jul 30, 2016·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Birte PlitzkoBernd Clement
Mar 25, 2017·International Journal of Molecular Sciences·Alejandro Chamizo-AmpudiaAngel Llamas
Mar 2, 2017·Redox Report : Communications in Free Radical Research·Natalia V PerkhulynVolodymyr I Lushchak
May 13, 2017·BioFactors·Angel LlamasEmilio Fernandez
May 12, 2018·FEBS Letters·Daniel Bender, Guenter Schwarz
Jun 6, 2018·Acta Crystallographica. Section F, Structural Biology Communications·Christian KubitzaAxel J Scheidig
Jan 16, 2013·Dalton Transactions : an International Journal of Inorganic Chemistry·Russ Hille
Jul 27, 2018·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Carsten GinselBernd Clement
Jun 7, 2019·Drug Metabolism Reviews·S Cyrus KhojastehDonglu Zhang
Jan 16, 2014·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Gudrun OttAntje Havemeyer
Apr 13, 2020·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Breeanna MintmierPartha Basu
Feb 7, 2014·The Journal of Biological Chemistry·Courtney E Sparacino-WatkinsMark T Gladwin
Jul 11, 2019·International Journal of Molecular Sciences·Jose María Miralles-RobledilloCarmen Pire

Related Concepts

ARC1 protein, human
Mitochondrial amidoxime reducing component 2, human
Mitochondrial amidoxime reducing component 1, human
Genes, Reiterated
Substrate Specificity
Mitochondrial Proteins

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.