Biochemical and structural properties of a low-temperature-active glycoside hydrolase family 43 β-xylosidase: Activity and instability at high neutral salt concentrations

Food Chemistry
Rui ZhangZunxi Huang

Abstract

β-Xylosidase, of the glycoside hydrolase family 43 from Bacillus sp. HJ14, was expressed in Escherichia coli. Recombinant β-xylosidase (rHJ14GH43) exhibited maximum activity at 25 °C, approximately 15, 45, and 88% of maximum activity at 0, 10, and 20 °C, respectively, and poor stability at temperatures over 20 °C. rHJ14GH43 showed moderate or high activity, but poor stability, in NaCl, KCl, NaNO3, KNO3, Na2SO4, and (NH4)2SO4 at concentrations from 3.0 to 30.0% (w/v). The crystal structure of rHJ14GH43 was resolved and showed higher structural flexibility due to fewer salt bridges and hydrogen bonds compared to mesophilic and thermophilic β-xylosidases. High structural flexibility is presumed to be a key factor for catalytic adaptations to low temperatures and high salt concentrations. Approximately one-third of the surface of rHJ14GH43 is positively charged, which may be the primary factor responsible for poor stability in high neutral salt environments.

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
May 16, 2002·Extremophiles : Life Under Extreme Conditions·Khawar Sohail SiddiquiTorsten Thomas
Mar 29, 2003·Extremophiles : Life Under Extreme Conditions·Michael Wainø, Kjeld Ingvorsen
Mar 24, 2004·Nature Reviews. Microbiology·Georges Feller, Charles Gerday
Apr 1, 1987·Applied and Environmental Microbiology·S F Lee, C W Forsberg
Jun 8, 2006·Annual Review of Biochemistry·Khawar Sohail Siddiqui, Ricardo Cavicchioli
Aug 10, 2006·Applied Microbiology and Biotechnology·Issam SmaaliMichael J O'Donohue
Jul 21, 2007·Nature Protocols·Manuela Benvenuti, Stefano Mangani
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Apr 13, 2010·Acta Crystallographica. Section D, Biological Crystallography·P EmsleyK Cowtan
Dec 6, 2011·Biotechnology Advances·Veeresh Juturu, Jin Chuan Wu
Dec 24, 2011·BMC Structural Biology·Alessandro SiglioccoloStefano Pascarella
Aug 31, 2012·Bioprocess and Biosystems Engineering·Vikash KumarT Satyanarayana
Apr 15, 2014·Folia Microbiologica·Junpei ZhouZunxi Huang
May 8, 2014·Applied Biochemistry and Biotechnology·Venkata Giridhar Poosarla, T S Chandra
Jul 24, 2015·Applied Biochemistry and Biotechnology·Douglas B JordanZdzislaw Wawrzak
Nov 6, 2015·Applied Biochemistry and Biotechnology·Adilson BosettoRita de Cássia Garcia Simão
Dec 22, 2015·Nature Communications·Andrew C WardenVictoria S Haritos
Jan 6, 2016·Applied and Environmental Microbiology·Keith MewisBernard Henrissat
Mar 28, 2018·International Journal of Biological Macromolecules·Daniella Romano de CarvalhoRosa Prazeres Melo Furriel
Jul 19, 2018·International Journal of Biological Macromolecules·Zheng-Wen CaiGuang-Ya Zhang
Nov 7, 2018·Nucleic Acids Research·Alex L MitchellRobert D Finn
Feb 20, 2019·Nature Biotechnology·José Juan Almagro ArmenterosHenrik Nielsen

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Citations

Nov 9, 2019·International Journal of Molecular Sciences·Ali RohmanNi Nyoman Tri Puspaningsih
Jul 30, 2021·Journal of Agricultural and Food Chemistry·Lijuan CaoZunxi Huang

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