Biochemical assay of collagen and elastin in the normal and varicose vein wall

The Journal of Surgical Research
M VenturiE Mussini

Abstract

Alterations of the connective tissue in the varicose vein wall have been noted by several investigators; however, the cause of the vein dilatation has still not been established. The aim of this study was to find a biochemical explanation to the development of varices by evaluating sensitive biochemical markers of collagen and elastin in the varicose vein wall. 4-L-Hydroxyproline (HYP), as a marker of collagen content, and desmosine (DES) and isodesmosine (IDES), as markers of elastin, were measured in 47 macroscopically dilated and 32 nondilated segments of 20 varicose saphenous veins collected from 20 patients with varices. The same measurements were made in 24 fragments of normal saphenous veins collected from 14 patients in whom the vein was removed to be used for graft procedures. HYP (collagen) and DES and IDES (elastin) were determined with a colorimetric method and HPLC, respectively. ANOVA test was used to compare mean values (+/- SD). HYP and collagen content were similar in varicose and normal veins. There was a significant reduction of both DES and IDES in dilated segments of varicose veins (P < 0.05 vs normal veins and nondilated segments); the ratio of elastin to collagen was lower in varicose than normal veins (P...Continue Reading

Citations

Sep 5, 2002·British Journal of Pharmacology·Chrystelle Cario-ToumaniantzGervaise Loirand
Jul 13, 2011·International Journal of Vascular Medicine·Zora HaviarováPeter Mráz
Feb 9, 2006·European Surgical Research. Europäische Chirurgische Forschung. Recherches Chirurgicales Européennes·S NomuraK Hamano
Mar 6, 2007·Journal of Vascular Research·Gemma PascualJulia Buján
Jan 15, 2015·Biosensors·Carlota SaldanhaAna Santos Silva-Herdade
Jul 20, 2002·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·M A Wali, R A Eid
Feb 25, 2003·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Mahmoud A Wali, Refaat A Eid
Feb 25, 2003·Journal of Smooth Muscle Research = Nihon Heikatsukin Gakkai Kikanshi·Mahmoud A WaliMohammad A Al-Homrany
Oct 28, 2011·Journal of Vascular and Interventional Radiology : JVIR·Rahmi OkluMichael T Watkins
Apr 28, 2009·The Journal of Surgical Research·Radoslaw KowalewskiMarek Gacko
Aug 7, 2007·Biochemical Pharmacology·Joseph D Raffetto, Raouf A Khalil
Oct 31, 2006·The Journal of Surgical Research·Joseph J NaoumChangyi Chen
Jun 11, 2009·Clinical and Experimental Dermatology·M KurzawskiM Droździk
Oct 23, 2009·The British Journal of Surgery·C S Lim, A H Davies
Jun 30, 2015·Journal of Pharmacopuncture·Pandi AnandakumarThiruvengadam Devaki
Jun 1, 1997·The Journal of Surgical Research·M VenturiA Peracchia
Dec 31, 1997·Journal of Vascular Surgery·N LabropoulosW H Baker
Aug 26, 2009·Journal of Vascular Surgery·Ying XiaoShenming Wang
Jul 5, 2003·Journal of Vascular Surgery·Kenneth J WoodsideGlenn C Hunter
Nov 12, 2010·Journal of Vascular Surgery·Alun H Davies, Chung S Lim
Jul 3, 2002·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·M CarmoP G Settembrini
Feb 16, 2006·Journal of Vascular Surgery·Henghui YinQing Liu
Oct 7, 2004·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·G L Sayer, P D C Smith
Apr 14, 2004·European Journal of Vascular and Endovascular Surgery : the Official Journal of the European Society for Vascular Surgery·A E AndroulakisI E Kallikazaros
Dec 3, 2014·Annals of Vascular Surgery·Oliwia Anna SegietRomuald Wojnicz
Oct 28, 2006·Angiology·Pamela Somers, Michiel Knaapen
Apr 12, 2011·Folia Histochemica Et Cytobiologica·Radoslaw KowalewskiKrzysztof Sobolewski
Aug 15, 2003·Journal of Biomedical Materials Research. Part a·Wafa M ElbjeiramiJennifer L West
Mar 10, 2001·The Journal of Pathology·C Badier-CommanderM P Jacob
Aug 17, 2012·Circulation. Cardiovascular Genetics·Muzaffar A AnwarAlun H Davies
Aug 25, 2015·Journal of Biomechanical Engineering·Semih E BezciGrace D O'Connell
Dec 13, 2005·Medical Principles and Practice : International Journal of the Kuwait University, Health Science Centre·W M RennoM Wali
Oct 14, 2014·The Open Urology & Nephrology Journal·Michelle K FittsYan-Ting Shiu
Sep 1, 2015·The Journal of Pharmacology and Experimental Therapeutics·Elisabeth MacColl, Raouf A Khalil
Nov 3, 2007·Vascular·Joseph J Naoum, Glenn C Hunter
Aug 26, 2003·Angiology·Patricia Sansilvestri-MorelTony J Verbeuren
Nov 19, 2003·Clinics in Plastic Surgery·Rachael H SchmedlenJennifer L West

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.