Biochemical assays of immobilized oligonucleotides with mass spectrometry

Langmuir : the ACS Journal of Surfaces and Colloids
Haim Tsubery, Milan Mrksich

Abstract

This paper reports the use of mass spectrometry to characterize oligonucleotides immobilized to the surfaces of biochips. Biotinylated oligonucleotides were immobilized to self-assembled monolayers that present a streptavidin layer and then treated with a complementary strand to present short duplexes. Treatment of the surface with 5-methoxysalicylic acid and ammonium citrate matrix allows the individual oligonucleotides to be observed by matrix-assisted laser desorption/iozation and time-of-flight mass spectrometry (MALDI-TOF MS). Examples are shown wherein this method is applied to assays of hybridization, of cleavage by a deoxyribozyme, of a dephosphorylation reaction, and of the adducts formed on treatment of DNA with cis-platin. This work provides an early example of the application of mass spectrometry to DNA biochips and may substantially expand the applications of the now common oligonucleotide arrays.

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Citations

Jul 22, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Na Young HaSang Yun Han
Oct 27, 2009·Nucleic Acids Research·Joohoon Kim, Milan Mrksich
Oct 30, 2009·Nucleic Acids Research·Kyu-Han KimKyu-Won Kim
Aug 19, 2014·Current Opinion in Biotechnology·Tristan de RondTrent Northen
Jan 22, 2011·Mass Spectrometry Reviews·Pawel L UrbanRenato Zenobi
Dec 18, 2009·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Dec 22, 2009·Integrative Biology : Quantitative Biosciences From Nano to Macro·William RowePhilip J R Day
Oct 6, 2019·SLAS Discovery·Shane M BukerP Ann Boriack-Sjodin
Aug 27, 2009·Langmuir : the ACS Journal of Surfaces and Colloids·Tae Kyung HaSang Yun Han
Jun 15, 2018·Journal of the American Chemical Society·Kazi Y HelalMilan Mrksich

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