PMID: 6691841Jan 1, 1984

Biochemical changes in endothelial cell monolayers induced by fibrin deposition in vitro

Arteriosclerosis : an Official Journal of the American Heart Association, Inc
R R Schleef, C Birdwell


Endothelial cells in vivo come into contact with fibrin during numerous physiological and pathological processes, but the nature of this interaction is poorly understood. The interaction of fibrin and endothelial cells was examined by incubating bovine aortic endothelial cell monolayers in vitro with fibrinogen (100 micrograms/ml) in medium supplemented with 10% calf serum. This treatment produced a layer of fibrin above the endothelial cell monolayer, which induced disorganization of the monolayer and remained associated with the monolayer even after washing. Endothelial cell pinocytosis of 14C-sucrose was elevated by 25% and 60% after incubation with fibrin for 8 hours and 2 days, respectively. Fibrin deposition on endothelial cell monolayers increased the rate of DNA synthesis (3H-thymidine incorporation) for 1 to 3 days by as much as 60% over controls. Concomitant with the lysis of fibrin above endothelial cells and the reorganization of the monolayer at Days 4 or 5 was a reduction of endothelial cell pinocytosis and DNA synthesis to control values. In contrast, there was no effect on the cell density at any time after incubation with fibrin. These results indicate that fibrin interacts with endothelial cells and induces a ...Continue Reading


Sep 7, 1976·Biochemical and Biophysical Research Communications·L Fésüs, K Laki
Sep 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·D J Loskutoff, T E Edgington
Aug 16, 1978·Molecular and Cellular Biochemistry·K C Robbins
Jul 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·C BirdwellG L Nicolson
May 1, 1978·British Medical Bulletin·N Woolf
Jun 1, 1978·Cell·R Ross, A Vogel
Jul 1, 1979·The Journal of Clinical Investigation·D J Loskutoff
Jan 1, 1979·Tissue & Cell·J KadishJ Folkman
Sep 1, 1979·Microvascular Research·S K WilliamsR C Wagner
Aug 1, 1979·Atherosclerosis·J Kadish
May 1, 1978·British Medical Bulletin·J R Mitchell
Jul 15, 1977·International Journal of Cancer. Journal International Du Cancer·E Engvall, E Ruoslahti
Nov 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·D GospodarowiczC Birdwell
Jan 1, 1976·Annals of the New York Academy of Sciences·N SimionescuG E Palade
Jun 1, 1975·Annals of Internal Medicine·R Matz
Apr 1, 1973·The Journal of Clinical Investigation·M B Bernik
Aug 1, 1974·Archives of Surgery·R A PeabodyC Eckert
Dec 8, 1972·Annals of the New York Academy of Sciences·K LakiJ Farrell
Mar 1, 1972·The Journal of Clinical Investigation·S NiewiarowskiJ F Mustard
Jan 1, 1973·Clinical Science·D OGSTONA S Douglas
Jan 1, 1983·Annals of the New York Academy of Sciences·S K Williams
Oct 28, 1982·Biochimica Et Biophysica Acta·S C Stamatoglou, J M Keller
Jul 1, 1952·The Journal of Pathology and Bacteriology·T CRAWFORD, C I LEVENE
Oct 1, 1957·American Journal of Clinical Pathology·H Z MOVAT, R H MORE
Jul 1, 1959·The Journal of Pathology and Bacteriology·A S TODD
Sep 1, 1963·The Journal of Experimental Medicine·P VASSALLIR T MCCLUSKEY
Dec 5, 1964·Lancet·D OGSTON, G M MCANDREW
Jan 1, 1948·The Journal of Pathology and Bacteriology·J B DUGUID


Jan 1, 1994·Chest·N HasegawaT A Raffin
Jul 15, 1986·Thrombosis Research·M RabaudH Bricaud
Apr 1, 1992·Journal of Cellular Physiology·S K LoA B Malik
Jan 1, 1989·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·K L KaplanS Solomon
Mar 1, 1986·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·B Weimar, U Delvos
Mar 1, 1990·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·E B SmithC Stirk
Jan 1, 1990·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·G Di Minno, M Mancini
Jan 28, 2019·Environmental Monitoring and Assessment·E E GoliaSp Vleioras
Dec 16, 1997·Scandinavian Journal of Clinical and Laboratory Investigation·B GambkeA Wefers
Nov 4, 2019·BMC Molecular and Cell Biology·Miriam CanteroRita Marchi

Related Concepts

Bos indicus
Cell Density
DNA, Double-Stranded
Antithrombin I

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.

Related Papers

Arteriosclerosis : an Official Journal of the American Heart Association, Inc
B Weimar, U Delvos
Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
P M Verschuren, J L Zevenbergen
© 2021 Meta ULC. All rights reserved