Biochemical characterisation of a hydrophobic ligand binding protein from the tapeworm Hymenolepis diminuta

International Journal for Parasitology
N SaghirJohn Barrett

Abstract

The cestode Hymenolepis diminuta contains an abundant, cytoplasmic, hydrophobic ligand, binding protein (H-HLBP). Studies with polarity sensitive probes suggest a single hydrophobic binding site, the results also indicate that the single tryptophan in the molecule (Trp41) is involved in ligand binding. Of the possible physiological ligands tested, only haematin and retinoids (retinol and retinoic acid) show appreciable binding in addition to fatty acids. H-HLBP also binds a range of anthelmintics, again with K(D) values in the nM range. The interaction of anthelmintics with hydrophobic binding proteins may be important in determining drug specificity and site of action and could have a role in the development of drug resistance.

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Citations

May 23, 2012·PLoS Neglected Tropical Diseases·Gonzalo ObalAna María Ferreira
Feb 9, 2005·Biochimica Et Biophysica Acta·Gustavo ChemaleArnaldo Zaha
Dec 3, 2014·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Valeria Silva-ÁlvarezAna María Ferreira
Oct 6, 2014·Prostaglandins, Leukotrienes, and Essential Fatty Acids·Gisela R FranchiniBetina Córsico

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