Biochemical characterisation of the chlamydial MurF ligase, and possible sequence of the chlamydial peptidoglycan pentapeptide stem

Archives of Microbiology
Delphine PatinD Blanot

Abstract

Chlamydiaceae are obligate intracellular bacteria that do not synthesise detectable peptidoglycan although they possess an almost complete arsenal of genes encoding peptidoglycan biosynthetic activities. In this paper, the murF gene from Chlamydia trachomatis was shown to be capable of complementing a conditional Escherichia coli mutant impaired in UDP-MurNAc-tripeptide:D-Ala-D-Ala ligase activity. Recombinant MurF from C. trachomatis was overproduced and purified from E. coli. It exhibited ATP-dependent UDP-MurNAc-X-γ-D-Glu-meso-A(2)pm:D-Ala-D-Ala ligase activity in vitro. No significant difference of kinetic parameters was seen when X was L-Ala, L-Ser or Gly. The L-Lys-containing UDP-MurNAc-tripeptide was a poorer substrate as compared to the meso-A(2)pm-containing one. Based on the respective substrate specificities of the chlamydial MurC, MurE, MurF and Ddl enzymes, a sequence L-Ala/L-Ser/Gly-γ-D-Glu-meso-A(2)pm-D-Ala-D-Ala is expected for the chlamydial pentapeptide stem, with Gly at position 1 being less likely.

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Citations

Jun 24, 2014·Nature Communications·Anna KlöcknerBeate Henrichfreise
Dec 3, 2013·Nature Communications·Martin PilhoferGrant J Jensen
Aug 21, 2015·Proceedings of the National Academy of Sciences of the United States of America·Mathanraj PackiamAnthony T Maurelli
Jun 19, 2012·Biochemical Pharmacology·Hélène BarreteauDidier Blanot
Feb 12, 2015·FEMS Microbiology Reviews·Nicolas JacquierGilbert Greub
Mar 30, 2018·Annual Review of Biochemistry·Atanas D RadkovMichael S VanNieuwenhze
Feb 26, 2020·Critical Reviews in Microbiology·Arlieke GitselsDaisy Vanrompay
Jun 17, 2020·Journal of Bacteriology·Scot P OuelletteJohn V Cox
May 23, 2013·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Vita MajceDavid I Roper
Oct 24, 2017·Antonie van Leeuwenhoek·Elena Rivas-Marín, Damien P Devos
Apr 3, 2021·Frontiers in Cellular and Infection Microbiology·Tobias C KunzMartin Fraunholz

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