Biochemical characterisation of the neuraminidase pool of the human gut symbiont Akkermansia muciniphila

Carbohydrate Research
Kun HuangJosef Voglmeir

Abstract

Since the isolation and identification of Akkermansia muciniphila one decade ago, much attention has been drawn to this gut bacterium due to its role in obesity and type 2 diabetes. This report describes the discovery and biochemical characterisation of all four putative neuraminidases annotated in the A. muciniphila genome. Recombinantly expressed candidate genes, which were designated Am0705, Am0707, Am1757 and Am2085, were shown to cover complementary pH ranges between 4.0 and 9.5. Temperature optima of the enzymes lay between 37 and 42 °C. All four enzymes were strongly inhibited by Cu(2+) and Zn(2+), and loss of activity after the addition of EDTA suggests that all neuraminidases, with the exception of Am0707, require divalent metal ions for their catalytic function. Chemoenzymatically synthesised α2,3- and α2,6-linked indoyl-sialosides were utilised to determine the regioselectivity and substrate promiscuity of the neuraminidases towards C5-modifications of sialic acids with N-acetyl-, N-glycolyl-, N-propionyl-, or hydroxyl-groups. The combination of simple purification procedures and good activities of some of the characterised neuraminidases makes them potentially interesting as tools in bioanalytical or industrial appl...Continue Reading

References

Feb 14, 1997·The Journal of Biological Chemistry·C M MilnerR D Campbell
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Aug 1, 2002·Proceedings of the National Academy of Sciences of the United States of America·Yoichiro KakugawaTaeko Miyagi
Apr 22, 2008·Nucleic Acids Research·A DereeperO Gascuel
Jul 18, 2008·Journal of Biochemistry·Taeko MiyagiKazuhiro Shiozaki
Sep 8, 2011·Infection and Immunity·Joseph J GillespieBruno W Sobral

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Citations

May 14, 2016·Cell Host & Microbe·Julia K GoodrichRuth E Ley
Mar 6, 2018·Glycobiology·Peter BothSabine L Flitsch
Apr 3, 2019·Clinical Pharmacology and Therapeutics·Anukriti SharmaJack A Gilbert
Feb 11, 2016·Biochemical Society Transactions·Nathalie JugeC David Owen
Apr 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Zhi-P CaiJosef Voglmeir
Dec 22, 2019·International Journal of Molecular Sciences·Xin MengHao Wu
Dec 26, 2018·Critical Reviews in Analytical Chemistry·Zuzana HľasováJaroslav Katrlík
Oct 13, 2020·Glycobiology·Andrew Bell, Nathalie Juge
Aug 18, 2020·Protein and Peptide Letters·Yuan-Yuan HanJosef Voglmeir
Jul 13, 2021·Frontiers in Cellular and Infection Microbiology·Chin-Hee SongYoung-Joon Surh

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