Biochemical characteristics and molecular mechanism of an exo-type alginate lyase VxAly7D and its use for the preparation of unsaturated monosaccharides

Biotechnology for Biofuels
Luyao TangFeng Han

Abstract

As the most abundant polysaccharide in brown algae, alginate has become a promising economical material for bioethanol production. Recently, exo-type alginate lyases have received extensive attention because the unsaturated monosaccharides produced by their degradation of alginate can be easily converted into 4-deoxy-l-erythro-5-hexoseulose uronate (DEH), a promising material for bioethanol production and biorefinery systems. In this study, we cloned and characterized an exo-type polysaccharide lyase family 7 (PL7) alginate lyase VxAly7D from the marine bacterium Vibrio xiamenensis QY104. Recombinant VxAly7D was most active at 30 °C and exhibited 21%, 46% and 90% of its highest activity at 0, 10 and 20 °C, respectively. Compared with other exo-type alginate lyases, recombinant VxAly7D was shown to be a bifunctional alginate lyase with higher specific activity towards sodium alginate, polyG and polyM (462.4 ± 0.64, 357.37 ± 0.53 and 441.94 ± 2.46 U/mg, respectively). A total of 13 μg recombinant VxAly7D could convert 3 mg sodium alginate to unsaturated monosaccharides in 1 min with a yield of 37.6%, and the yield reached 95% in 1 h. In addition, the three-dimensional structure of VxAly7D was modelled using the crystal structure ...Continue Reading

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Citations

Aug 4, 2020·International Journal of Biological Macromolecules·Danyang ChengXiangzhao Mao
Sep 2, 2020·International Journal of Biological Macromolecules·Zhelun ZhangFeng Han
Oct 11, 2020·International Journal of Biological Macromolecules·Yujiao LiFeng Han
Jun 14, 2021·Carbohydrate Polymers·Ushasree Mrudulakumari VasudevanEun Yeol Lee
Aug 28, 2021·International Journal of Molecular Sciences·Fengchao ZhangWengong Yu

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Datasets Mentioned

BETA
MN704375

Methods Mentioned

BETA
gel filtration
PCR
electrophoresis

Software Mentioned

PyMOL
MEGA
Verify3D
ClustalW
BLASTp
Compute pI / Mw

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