PMID: 9437664Jan 23, 1998Paper

Biochemical characteristics of gamma-glutamyl transpeptidase in capillaries from entorhinohippocampal complex of quinolinate-lesioned rat brain

Molecular and Chemical Neuropathology
F StastnýV Lisý

Abstract

Quinolinic acid (QUIN) is an endogenous excitotoxic agonist of the N-methyl-D-aspartate (NMDA) type of glutamate receptor, which causes slowly progressing degeneration of vulnerable neurons in some brain regions. Using changes in the activity of membrane-bound gamma-glutamyl transpeptidase (GGT) as a marker of cell damage, we found a significant decrease of this enzyme activity, which was preferentially located in the ipsilateral hippocampal formation and entorhinal cortex, 4 d after the unilateral intracerebroventricular (icv) injection of 0.5 mumol QUIN. The dose of QUIN divided into two half-doses injected bilaterally led to a symmetrical decline of GGT activity in hippocampal areas. The lesion was characterized by a suppression of GGT activity in hippocampal and entorhinal capillaries, corresponding to 60 and 81% of their initial value, respectively, but no significant changes were ascertained in synaptosomal membranes. The changes in the activity of capillary GGT were associated with the decrease of apparent maximal velocity Vmaxapp, whereas apparent Michaelis constant K(m)app (0.69-0.79 mM) remained unaffected. In the nonlesioned brain, concanavalin A (Con A) affinity chromatography revealed five glycoforms of synaptosoma...Continue Reading

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Citations

May 17, 2005·Brain Research. Molecular Brain Research·V MaresA Sedo
Jan 5, 2002·Neuroscience Letters·Lioudmila PlissFrantisek St'astný
Jan 4, 2001·Brain Research Bulletin·F St'astnýD Jezová
Jun 16, 1997·Toxicology Letters·R Barroso-MoguelC Ríos
Mar 1, 2016·Neuropharmacology·Nicholas H HuntHelen J Ball

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