Biochemical characterization and crystal structure of Synechocystis arogenate dehydrogenase provide insights into catalytic reaction

Structure
Pierre LegrandMichel Matringe

Abstract

The extreme diversity in substrate specificity, and in the regulation mechanism of arogenate/prephenate dehydrogenase enzymes in nature, makes a comparative structural study of these enzymes of great interest. We report here on the biochemical and structural characterization of arogenate dehydrogenase from Synechocystis sp. (TyrAsy). This work paves the way for the understanding of the structural determinants leading to diversity in substrate specificity, and of the regulation mechanisms of arogenate/prephenate dehydrogenases. The overall structure of TyrAsy in complex with NADP was refined to 1.6 A. The asymmetric unit contains two TyrAsy homodimers, with each monomer consisting of a nucleotide binding N-terminal domain and a particularly unique alpha-helical C-terminal dimerization domain. The substrate arogenate was modeled into the active site. The model of the ternary complex enzyme-NADP-arogenate nicely reveals at the atomic level the concerted mechanism of the arogenate/prephenate dehydrogenase reaction.

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Citations

May 5, 2012·Annual Review of Plant Biology·Hiroshi Maeda, Natalia Dudareva
Mar 7, 2008·Microbiology and Molecular Biology Reviews : MMBR·Carol A BonnerRoy A Jensen
Mar 13, 2009·The Journal of Biological Chemistry·Warren SunDinesh Christendat
Oct 15, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Hsiu Ju ChiuIan A Wilson
Sep 12, 2012·BMC Plant Biology·Fiammetta AlagnaLuciana Baldoni
Jul 30, 2011·International Journal of Biological Macromolecules·Hyung-Keun KuSoo Jae Lee
Nov 18, 2014·Nature Chemical Biology·Craig A SchenckHiroshi A Maeda
Jun 19, 2010·Journal of Experimental Botany·David R HoldingBrian A Larkins
Mar 31, 2018·Chembiochem : a European Journal of Chemical Biology·Cynthia K Holland, Joseph M Jez
Nov 21, 2018·The Plant Journal : for Cell and Molecular Biology·Marcos V V de OliveiraHiroshi A Maeda
Sep 29, 2019·The Journal of Biological Chemistry·Hiroshi A Maeda
Jul 4, 2017·Nature Chemical Biology·Craig A SchenckHiroshi A Maeda
Feb 27, 2018·Phytochemistry·Craig A Schenck, Hiroshi A Maeda
Oct 16, 2021·Journal of Agricultural and Food Chemistry·Haojie JinYujie Fu

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