Biochemical Characterization and Molecular Modeling of Pancreatic Lipase from a Cartilaginous Fish, the Common Stingray (Dasyatis pastinaca)

Applied Biochemistry and Biotechnology
Emna BouchaalaAhmed Fendri

Abstract

In order to identify fish enzymes displaying novel biochemical properties, we have chosen the common stingray (Dasyatis pastinaca), one of the most primitive living jawed aquatic vertebrates as a starting biological material to purify a lipase. A stingray pancreatic lipase (SPL) was purified from delipidated pancreatic powder. The SPL molecular weight was around 55 kDa which is slightly higher than that of known classical pancreatic lipases (50 kDa). This increase in the molecular weight was due to glycosylation. Like classic pancreatic lipases, SPL was found to be much more active on short-chain triacylglycerols than on long-chain ones. Natural detergents act as inhibitors of the SPL activity. This inhibition can be reversed by the addition of stingray colipase. Starting from total pancreatic messenger RNAs (mRNAs), partial stingray pancreatic lipase complementary DNA (cDNA) was synthesized by reverse transcriptase-polymerase chain reaction (RT-PCR) and cloned into the PGEM-T vector. Partial amino acid sequence of the SPL was homologous to that of Japanese eel, porcine, and human pancreatic lipases. A 3D structure model of the sequenced part of SPL was built using the 3D structure of porcine pancreatic lipase as template, sinc...Continue Reading

References

Feb 22, 1977·Biochimica Et Biophysica Acta·A De CaroO Guy
Apr 1, 1992·Protein Engineering·D L OllisJ Schrag
Mar 4, 1992·Biochimica Et Biophysica Acta·D R GjellesvikB T Walther
Dec 18, 1989·Biochimica Et Biophysica Acta·Y GargouriR Verger
Apr 15, 1986·European Journal of Biochemistry·Y GargouriR Verger
Dec 5, 1986·Biochimica Et Biophysica Acta·Y GargouriR Verger
Sep 2, 1968·Biochimica Et Biophysica Acta·G Benzonana, P Desnuelle
Sep 11, 1984·Biochimica Et Biophysica Acta·B SternbyU Hellman
Dec 29, 1981·Biochimica Et Biophysica Acta·J de CaroM Rovery
Feb 10, 1993·Biochimica Et Biophysica Acta·C S Wang, J A Hartsuck
Jan 1, 1997·Methods in Enzymology·F FerratoR Verger
Jan 1, 1997·Methods in Enzymology·J D Schrag, M Cygler
Nov 7, 1998·Biochimica Et Biophysica Acta·F CarrièreR Verger
Mar 23, 2000·Biochimie·A SayariY Gargouri
Jul 2, 2002·Journal of Lipid Research·Howard Wong, Michael C Schotz
Nov 23, 2005·Bioinformatics·Konstantin ArnoldTorsten Schwede
Jun 20, 2006·Archives of Biochemistry and Biophysics·Ahmed FendriYoussef Gargouri
Sep 12, 2007·Bioinformatics·M A LarkinD G Higgins
Oct 22, 2008·Nucleic Acids Research·Florian KieferTorsten Schwede
Jan 23, 2010·Bioresource Technology·Slim Cherif, Youssef Gargouri
Apr 3, 2013·International Journal of Biological Macromolecules·Nabil SmichiAhmed Fendri

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