Biochemical characterization of a phospholipase A2 homologue from the venom of the social wasp Polybia occidentalis

The Journal of Venomous Animals and Toxins Including Tropical Diseases
Rafaela Diniz-SousaAndreimar M Soares

Abstract

Wasp venoms constitute a molecular reservoir of new pharmacological substances such as peptides and proteins, biological property holders, many of which are yet to be identified. Exploring these sources may lead to the discovery of molecules hitherto unknown. This study describes, for the first time in hymenopteran venoms, the identification of an enzymatically inactive phospholipase A2 (PLA2) from the venom of the social wasp Polybia occidentalis. P. occidentalis venom was fractioned by molecular exclusion and reverse phase chromatography. For the biochemical characterization of the protein, 1D and 2D SDS-PAGE were performed, along with phospholipase activity assays on synthetic substrates, MALDI-TOF mass spectrometry and sequencing by Edman degradation. The protein, called PocTX, was isolated using two chromatographic steps. Based on the phospholipase activity assay, electrophoresis and mass spectrometry, the protein presented a high degree of purity, with a mass of 13,896.47 Da and a basic pI. After sequencing by the Edman degradation method, it was found that the protein showed a high identity with snake venom PLA2 homologues. This is the first report of an enzymatically inactive PLA2 isolated from wasp venom, similar to sn...Continue Reading

References

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Methods Mentioned

BETA
size exclusion chromatography
reverse phase chromatography
electrophoresis

Software Mentioned

BLAST
PocTX

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