Biochemical characterization of Arabidopsis wild-type and mutant phytochrome B holoproteins

The Plant Cell
T D Elich, J Chory

Abstract

Although phytochrome B (phyB) plays a particularly important role throughout the life cycle of a plant, it has not been studied in detail at the molecular level due to its low abundance. Here, we report on the expression, assembly with chromophore, and purification of epitope-tagged Arabidopsis phyB. In addition, we have reconstructed two missense mutations, phyB-4 and phyB-101, isolated in long hypocotyl screens. We show that mutant proteins phyB-4 and phyB-101 exhibit altered spectrophotometric and biochemical properties relative to the wild-type protein. In particular, we demonstrate that phyB-101 Pfr exhibits rapid nonphotochemical (dark) reversion to Pr that results in a lower photoequilibrium level of the active Pfr form. We conclude that this occurs in vivo as well because phyB-101 mutants are shown to lack an end-of-day-far-red hypocotyl elongation response that requires a stable Pfr species. We propose that this Pfr instability may be the primary molecular mechanism underlying the phyB-101 mutant phenotype.

References

Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J A WahleithnerJ C Lagarias
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·D Wagner, P H Quail
Aug 25, 1995·The Journal of Biological Chemistry·T KunkelE Schäfer
Dec 1, 1994·Plant Molecular Biology·T D Elich, J Chory
Aug 1, 1993·European Journal of Biochemistry·T KunkelE Schäfer
Dec 1, 1995·Plant Molecular Biology·D M LagariasJ C Lagarias
Jul 23, 1996·Proceedings of the National Academy of Sciences of the United States of America·T ShinomuraM Furuya
Apr 1, 1997·Photochemistry and Photobiology·J T Murphy, J C Lagarias

❮ Previous
Next ❯

Citations

Nov 26, 1999·The Plant Journal : for Cell and Molecular Biology·J F Martínez-GarcíaP H Quail
Aug 30, 2008·Proceedings of the National Academy of Sciences of the United States of America·Daisy W LeungMichael K Rosen
Jun 28, 2000·Proceedings of the National Academy of Sciences of the United States of America·L Krall, J W Reed
Oct 12, 1999·Plant Physiology·L HennigE Schäfer
May 4, 2006·Annual Review of Plant Biology·Nathan C RockwellJ Clark Lagarias
Nov 24, 2011·PloS One·Fa-Qiang WuYong-Fu Fu
May 15, 2012·The Arabidopsis Book·Jorge J Casal
Jan 1, 2004·The Arabidopsis Book·Haiyang Wang, Xing Wang Deng
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·H HanzawaM Furuya
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·M M NeffJ Chory
Feb 27, 2014·Journal of Experimental Botany·Keara A FranklinKaren J Halliday
Nov 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Meng ChenJoanne Chory
Aug 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tilman LamparterBerta Esteban
Apr 26, 2003·The Plant Journal : for Cell and Molecular Biology·Robert A SharrockLynn Goosey
Nov 2, 2014·Ecology and Evolution·David C Gibbs, Kathleen Donohue
Sep 1, 2004·Current Opinion in Plant Biology·Isabelle SchepensChristian Fankhauser
Jan 1, 2015·Nature Plants·Cornelia KloseChristian Fleck
Jun 12, 2013·Cell Reports·Kazumasa NitoJoanne Chory
Jan 9, 2001·Seminars in Cell & Developmental Biology·C Fankhauser
Dec 23, 2016·The Plant Journal : for Cell and Molecular Biology·Martina LegrisJorge J Casal
Dec 13, 2016·Plant, Cell & Environment·András VicziánFerenc Nagy
Jun 5, 2007·Journal of Experimental Botany·Virtudes Mira-RodadoKlaus Harter
Mar 30, 2001·The Journal of Biological Chemistry·C Fankhauser
Dec 22, 2017·Nature Communications·Beatrix EnderleAndreas Hiltbrunner
Dec 29, 1998·The Journal of Biological Chemistry·K EichenbergE Schäfer
Apr 6, 2019·Proceedings of the National Academy of Sciences of the United States of America·He HuangDmitri A Nusinow
Feb 4, 2021·International Journal of Molecular Sciences·Rocio Quian-Ulloa, Claudia Stange

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.