Biochemical characterization of Borrelia burgdorferi's RecA protein

PloS One
Shu Hui HuangKerri Kobryn

Abstract

RecA plays key roles in DNA recombination, replication and repair. Mutation of recA in the Lyme disease spirochete, Borrelia burgdorferi, fails to produce some of the phenotypes expected from study of recA mutation in other organisms. 'Missing' recA phenotypes include a lack of growth or viability effects, including in the presence of DNA damage, and a lack of a role in vlsE antigenic variation and infectivity. We present a purification and biochemical characterization of recombinant B. burgdorferi RecA protein. We find that B. burgdorferi RecA displays the expected properties of being a DNA-dependent ATPase, of having an intrinsic binding preference for ssDNA over dsDNA enhanced by ATP binding, of promoting DNA pairing and strand exchange reactions and of having a detectable coprotease activity with E. coli LexA repressor. DNA pairing and strand exchange reactions promoted by B. burgdorferi RecA show an unusually strong dependence upon the presence of the cognate ssDNA binding protein (SSB) but are very sensitive to inhibition by SSB when the ssDNA was prebound by SSB. This indicates B. burgdorferi RecA may have an enhanced requirement for recombinational mediators to promote RecA-SSB exchange, despite the absence of homologue...Continue Reading

References

Apr 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T ShibataC M Radding
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·J P MenetskiS C Kowalczykowski
Jan 25, 1985·The Journal of Biological Chemistry·F R BryantI R Lehman
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J W Little
May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·K MuniyappaC M Radding
Jan 1, 1984·Cold Spring Harbor Symposia on Quantitative Biology·P Howard-FlandersE H Egelman
Jun 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J W LittleD W Mount
Mar 8, 1996·The Journal of Biological Chemistry·Q ShanR B Inman
May 17, 1996·The Journal of Biological Chemistry·W M Rehrauer, S C Kowalczykowski
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·A V Mazin, S C Kowalczykowski
Nov 8, 1996·The Journal of Biological Chemistry·P Sung, S A Stratton
Mar 28, 1998·The EMBO Journal·A V Mazin, S C Kowalczykowski
Mar 28, 1998·Gene·C G BlackJ K Davies
Jun 19, 1998·Frontiers in Bioscience : a Journal and Virtual Library·P R BiancoS C Kowalczykowski
Dec 10, 1999·Microbiology and Molecular Biology Reviews : MMBR·A Kuzminov
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·J A Solinger, W D Heyer
Jul 24, 2001·Journal of Bacteriology·C H EggersD S Samuels
Aug 10, 2002·The Journal of Biological Chemistry·Aimee L EgglerMichael M Cox
Feb 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·A J CLARK, A D MARGULIES
Nov 18, 2003·Annual Review of Genetics·Justin Courcelle, Philip C Hanawalt
Feb 13, 2004·Bioinformatics·Michele ClampGeoffrey J Barton
Apr 3, 2004·Journal of Bacteriology·Dionysios LiverisIra Schwartz
Jul 9, 2008·Infection and Immunity·Dionysios LiverisIra Schwartz
Aug 30, 2008·Cellular and Molecular Life Sciences : CMLS·M ButalaS J W Busby
Oct 22, 2008·Critical Reviews in Biochemistry and Molecular Biology·Robert D SheredaJames L Keck
Mar 2, 2011·PloS One·Elizabeth A StohlH Steven Seifert
May 24, 2011·Critical Reviews in Biochemistry and Molecular Biology·Jie LiuScott W Morrical

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Citations

Mar 3, 2020·Frontiers in Microbiology·Duo-Hong ShengYue-Zhong Li

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Datasets Mentioned

BETA
AAC66507
AAC66492.1

Methods Mentioned

BETA
electrophoretic mobility shift assay

Software Mentioned

Protein Figure Manipulation Suite

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