Biochemical characterization of cathepsin D from the mussel Lamellidens corrianus

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
Ashapogu Venugopal, Nadimpalli Siva Kumar

Abstract

A lysosomal cathepsin D (EC 3.4.23.5) was purified to homogeneity from the soft tissues of the fresh water mussel (Lamellidens corrianus) by pepstatin A affinity chromatography. The purified enzyme is a glycoprotein and migrates as a single protein species in native PAGE and shows a single band in SDS-PAGE corresponding to a molecular mass of ~43 kDa. Under both these conditions cathepsin D hydrolyzes hemoglobin as shown by zymogram analysis. The purified enzyme cross-reacts with an antiserum to purified starfish (Asterias rubens) cathepsin D. Additionally, the enzyme was recognized by the starfish lysosomal enzyme targeting receptors (mannose 6-phosphate receptors: MPR 300 and 46) in ligand blot analysis. The KM value of the purified enzyme with hemoglobin is 1.5mM. pH and temperature optimum for the enzyme are 3.5 and 60 °C respectively.

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Citations

Apr 6, 2019·Protein and Peptide Letters·Cadena-Cadena FranciscoRivero-Espejel Ignacio Alfredo
Jun 9, 2018·The Protein Journal·Aravindakshan Ajith Kumar, Nadimpalli Siva Kumar
Feb 23, 2020·International Journal of Biological Macromolecules·Rohit Sai Reddy KonadaSiva Kumar Nadimpalli

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