Biochemical Characterization of Fungus Isolated during In vitro Propagation of Bambusa balcooa

Pharmacognosy Magazine
Bhawna TyagiAshutosh Dubey

Abstract

Bambusa balcooa (Poaceae: Bambusoideae) is a multipurpose bamboo species, which is native of the Indian subcontinent. B. balcooa is regarded as one of the best species for scaffolding and building purposes because of its strong culm. Other uses include paper pulp, handicrafts, and products of the wood chip industry. Due to these various uses in industries, this species has been identified as one of the priority bamboos by the National Bamboo Mission. This study is designed to analyze the identification of fungus and develop the strategy to eliminate the contamination during in vitro establishment of B. balcooa through nodal part. Fungus contamination is a problem which is encountered during in vitro establishment of B. balcooa cultures. In the present study, fungus contamination from in vitro cultured plant has been isolated and subjected to partial sequence analysis of the 18S rRNA gene to identify the fungus strain. Experiments were designed to develop a strategy for removal of the fungus contamination with the help of antifungal compounds and commercial antimicrobial supplement supplied by HiMedia. Fusarium equiseti was identified as endophytic fungus. It was observed that antimicrobial supplement at concentration of 500 μl/...Continue Reading

Citations

Apr 16, 2021·Frontiers in Microbiology·Abdoulaye SoumareMame Ourèye Sy

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

MEGA
BLAST

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