Biochemical characterization of human erythrocytes fractionated by counter-current distribution in aqueous polymer two-phase systems

Journal of Chromatography. a
M PinillaJ Luque


The fractionation of normal human erythrocytes by counter-current distribution (CCD) in charge-sensitive dextran-polyethylene glycol two-phase systems was confirmed and extended to red blood cells from heterozygous beta-thalassaemic patients. The differences between the distribution profiles of normal (homogeneous) and abnormal (heterogeneous) red blood cells reflect their different surface-charge properties. As suggested by the decline of membrane sialic acid released after neuraminidase treatment and the specific activities of two age-dependent enzymes (membrane acetylcholinesterase and intracellular pyruvate kinase) in the distribution profiles (from the left- to the right-hand side fractions), the fractionation seems to be according to red blood cell age. A constancy of the 2,3-bisphosphoglycerate level was observed in ageing red blood cells.


Oct 22, 2008·Biomedical Microdevices·Jeffrey R Soohoo, Glenn M Walker
Jun 22, 2019·Journal of Colloid and Interface Science·Morteza JeyhaniScott S H Tsai

Related Concepts

Bisphosphoglycerate Mutase
Cell Separation
Countercurrent Chromatography
Diphosphoglyceric Acids
Erythrocyte Survival
Mean Corpuscular Hemoglobin Concentration Determination
Red Cell Ghost

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