Biochemical characterization of long-term culture of the Swarm rat chondrosarcoma chondrocytes in agarose

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
A M KucharskaJames H Kimura

Abstract

Difficulty in maintaining phenotypic stability of the Swarm rat chondrosarcoma in long-term monolayer cultures has prompted investigation of alternative conditions that would enable extended maintenance of these cells, permitting use of the tumor as a model system for the long-term study of proteoglycan metabolism. Morphological analysis of the growth of the chondrosarcoma chondrocytes in agarose has shown stability of the culture over a 20 day period with respect to the ability of the cells to proliferate and synthesize an Alcian blue-positive extracellular matrix. The present study confirms these findings through analysis of the growth characteristics of the culture and the pattern of proteoglycan and collagen synthesis. The chondrocytes actively synthesize a proteoglycan-rich matrix at a rate dependent on the initial plating density and concentration of serum in the culture medium. These factors similarly affect the proliferative capabilities of the culture as demonstrated by the growth curves obtained at different culture conditions. During 20 days in culture, the cells synthesize an aggregating chondroitin sulfate proteoglycan and collagen type II, typical of cartilage and this chondrosarcoma. In addition, small molecular ...Continue Reading

References

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Citations

Mar 29, 2002·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Masahiro YonekawaHisashi Iwata
Apr 21, 2016·Matrix Biology : Journal of the International Society for Matrix Biology·Yi HuangWarren Knudson
Aug 14, 2001·The Anatomical Record·Y H AnR J Friedman
Jul 23, 2003·Journal of Cellular Biochemistry·Karen B King, James H Kimura
Mar 1, 1994·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·L A MacGinitieA J Grodzinsky
Nov 1, 1992·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M D BuschmannE B Hunziker
Jan 7, 1999·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·P E Di CesareA R Amin
Jul 26, 2013·The Journal of Biological Chemistry·Liliana MellorWarren Knudson
Apr 27, 2001·The Journal of Biological Chemistry·A AzizanP J Neame
Feb 19, 2019·Archives of Biochemistry and Biophysics·Kenya TerabeWarren Knudson
Jul 24, 1998·Biochemical and Biophysical Research Communications·S F McClureJ McClure

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