Biochemical characterization of peptidylarginine deiminase-like orthologs from thermotolerant Emericella dentata and Aspergillus nidulans

Enzyme and Microbial Technology
Ashraf S A El-SayedMahmoud Z Sitohy

Abstract

Peptidylarginine deiminases (PADs) are a group of hydrolases, mediating the deimination of peptidylarginine residues into peptidyl-citrulline. Equivocal protein citrullination by PADs of fungal pathogens has a strong relation to the progression of multiple human diseases, however, the biochemical properties of fungal PADs remain ambiguous. Thus, this is the first report exploring the molecular properties of PAD from thermotolerant fungi, to imitate the human temperature. The teleomorph Emericella dentata and anamorph Aspergillus nidulans have been morphologically and molecularly identified, with observed robust growth at 37-40 °C, and strong PAD productivity. The physiological profiles of E. dentata and A. nidulans for PADs production in response to carbon, nitrogen sources, initial medium pH and incubation temperature were relatively identical, emphasizing the taxonomical proximity of these fungal isolates. PADs were purified from E. dentata and A. nidulans with apparent molecular masses 41 and 48 kDa, respectively. The peptide fingerprints of PADs from E. dentata and A. nidulans have been analyzed by MALDI-TOF/MS, displaying a higher sequence similarity to human PAD4 by 18% and 31%, respectively. The conserved peptide sequenc...Continue Reading

Citations

Jan 16, 2020·Biology·Richard A PhillipsSigrun Lange
Jul 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ashraf S A El-SayedBasel Sitohy
Jul 8, 2020·International Journal of Molecular Sciences·Elif Damla ArisanSigrun Lange
Aug 1, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ashraf S A El-SayedMahmoud Z Sitohy
Jan 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ashraf S A El-SayedBasel Sitohy
Oct 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ashraf El-SayedNashwa El-Gazzar
Oct 8, 2020·Heliyon·Manal T El Sayed, Ashraf S A El-Sayed
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mohamed RaafatManal T El-Sayed
Apr 27, 2021·Frontiers in Plant Science·Claudius MarondedzeChris Gehring

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.