Abstract
Peptidylarginine deiminases (PADs) are a group of hydrolases, mediating the deimination of peptidylarginine residues into peptidyl-citrulline. Equivocal protein citrullination by PADs of fungal pathogens has a strong relation to the progression of multiple human diseases, however, the biochemical properties of fungal PADs remain ambiguous. Thus, this is the first report exploring the molecular properties of PAD from thermotolerant fungi, to imitate the human temperature. The teleomorph Emericella dentata and anamorph Aspergillus nidulans have been morphologically and molecularly identified, with observed robust growth at 37-40 °C, and strong PAD productivity. The physiological profiles of E. dentata and A. nidulans for PADs production in response to carbon, nitrogen sources, initial medium pH and incubation temperature were relatively identical, emphasizing the taxonomical proximity of these fungal isolates. PADs were purified from E. dentata and A. nidulans with apparent molecular masses 41 and 48 kDa, respectively. The peptide fingerprints of PADs from E. dentata and A. nidulans have been analyzed by MALDI-TOF/MS, displaying a higher sequence similarity to human PAD4 by 18% and 31%, respectively. The conserved peptide sequenc...Continue Reading
Citations
Jan 16, 2020·PloS One·Olena MahnevaWilliam W Ja
Jan 16, 2020·Biology·Richard A PhillipsSigrun Lange
Jul 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ashraf S A El-SayedBasel Sitohy
Jul 8, 2020·International Journal of Molecular Sciences·Elif Damla ArisanSigrun Lange
Aug 1, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ashraf S A El-SayedMahmoud Z Sitohy
Nov 2, 2019·International Journal of Molecular Sciences·Matthew E PamenterSigrun Lange
Apr 25, 2020·International Journal of Molecular Sciences·Michael F CriscitielloSigrun Lange
May 16, 2020·Frontiers in Immunology·Michael F CriscitielloSigrun Lange
Jan 16, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ashraf S A El-SayedBasel Sitohy
Dec 2, 2020·Biology·Timothy J BowdenSigrun Lange
Oct 17, 2019·Enzyme and Microbial Technology·Ashraf S A El-SayedBasel Sitohy
Jan 21, 2021·International Journal of Molecular Sciences·Bergljót MagnadóttirSigrun Lange
Oct 24, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ashraf El-SayedNashwa El-Gazzar
Oct 8, 2020·Heliyon·Manal T El Sayed, Ashraf S A El-Sayed
Feb 28, 2020·Biochimie·Bergljót MagnadóttirSigrun Lange
Apr 27, 2020·Developmental and Comparative Immunology·Timothy J BowdenSigrun Lange
Mar 4, 2021·Biology·Árni KristmundssonSigrun Lange
Jul 31, 2020·Fish & Shellfish Immunology·Timothy J BowdenSigrun Lange
Apr 4, 2021·Biology·Stefania D'AlessioSigrun Lange
Apr 4, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Hanaa Salah MaamounAshraf S A El-Sayed
May 1, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Mohamed RaafatManal T El-Sayed
Apr 27, 2021·Frontiers in Plant Science·Claudius MarondedzeChris Gehring
May 25, 2021·Biotechnology Reports·Sobhy S Abdel-FatahAshraf S El-Sayed
Aug 7, 2021·Developmental and Comparative Immunology·Jonathan P RastSigrun Lange