Biochemical characterization of the aba2 and aba3 mutants in Arabidopsis thaliana

Plant Physiology
S H SchwartzJan A D Zeevaart

Abstract

Abscisic acid (ABA)-deficient mutants in a variety of species have been identified by screening for precocious germination and a wilty phenotype. Mutants at two new loci, aba2 and aba3, have recently been isolated in Arabidopsis thaliana (L.) Hynh. (K.M. Léon-Kloosterziel, M. Alvarez-Gil, G.J. Ruijs, S.E. Jacobsen, N.E. Olszewski, S.H. Schwartz, J.A.D. Zeevaart, M. Koornneef [1996] Plant J 10: 655-661), and the biochemical characterization of these mutants is presented here. Protein extracts from aba2 and aba3 plants displayed a greatly reduced ability to convert xanthoxin to ABA relative to the wild type. The next putative intermediate in ABA synthesis, ABA-aldehyde, was efficiently converted to ABA by extracts from aba2 but not by extracts from aba3 plants. This indicates that the aba2 mutant is blocked in the conversion of xanthoxin to ABA-aldehyde and that aba3 is impaired in the conversion of ABA-aldehyde to ABA. Extracts from the aba3 mutant also lacked additional activities that require a molybdenum cofactor (Moco). Nitrate reductase utilizes a Moco but its activity was unaffected in extracts from aba3 plants. Moco hydroxylases in animals require a desulfo moiety of the cofactor. A sulfido ligand can be added to the Moco...Continue Reading

References

Jan 1, 2002·The Arabidopsis Book·Ruth R Finkelstein, Christopher D Rock
Mar 20, 2013·Plant Cell Reports·Mattia DonàAlma Balestrazzi
Dec 7, 2007·Journal of Plant Research·Vanessa MelhornTomokazu Koshiba
Oct 16, 2012·Plant Molecular Biology·Maryam ZarepourFlorian Bittner
Apr 9, 2005·Trends in Plant Science·Alexander R MoiseKrzysztof Palczewski
Apr 25, 2000·Plant Science : an International Journal of Experimental Plant Biology· Katalin Barabás N Herman Lips S
Jan 24, 2002·Trends in Plant Science·Mitsunori Seo, Tomokazu Koshiba
Nov 24, 1999·Trends in Plant Science·A J Cutler, J E Krochko
Aug 6, 2000·The Plant Journal : for Cell and Molecular Biology·A J ThompsonI B Taylor
Sep 6, 2000·The Plant Journal : for Cell and Molecular Biology·M SeoT Koshiba
Jun 6, 2002·The Plant Journal : for Cell and Molecular Biology·S MerlotJerome Giraudat
Aug 8, 2002·The Plant Journal : for Cell and Molecular Biology·Moshe SagiRobert Fluhr
Sep 29, 2009·Journal of Experimental Botany·Gaston ZollaSimon Barak
Oct 27, 2004·Plant Physiology·Rejane L Guimarães, Henrik U Stotz
Jul 1, 1997·Plant Physiology·S Merlot, Jerome Giraudat
Aug 13, 2002·The Plant Cell·Miguel González-GuzmánPedro L Rodríguez
Sep 11, 2001·The Plant Cell·Nancy A Eckardt
Jul 6, 2006·Applied and Environmental Microbiology·Verena SiewersPaul Tudzynski
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·Jeffrey Leung, Jerome Giraudat
Dec 9, 2008·BMC Plant Biology·Oumaya Bouchabke-CoussaMylène Durand-Tardif
Mar 17, 2011·PloS One·Valérie LefebvreMylène Durand-Tardif
Mar 29, 2014·Plant Physiology·Margaret E WilsonElizabeth S Haswell
Apr 13, 2013·Journal of Experimental Botany·David A KorasickLucia C Strader
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·X Qin, Jan A D Zeevaart
Oct 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·M SeoT Koshiba
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·B C TanD R McCarty
Jul 12, 2014·Journal of Plant Physiology·Akira EndoYutaka Sato
Jul 7, 2009·Annual Review of Plant Biology·Jan A D Zeevaart
Jan 13, 2015·Molecular Plant·Nazia NisarBarry J Pogson
May 16, 2009·Trends in Plant Science·Jurriaan TonBrigitte Mauch-Mani

Citations

Mar 14, 1972·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·H F Taylor, R S Burden
Oct 1, 1996·The Plant Journal : for Cell and Molecular Biology·K M Léon-KloosterzielM Koornneef

Related Concepts

Genes, Plant
Carotenoids
Xanthoxin
Sesquiterpenes
Germination
Molybdenum cofactor
Metalloproteins
Nitrate Reductases
Aldehyde Oxidoreductases
Xanthoxin Dehydrogenase Activity

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